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Formalin-fixed, paraffin-embedded human HCV-infected liver stained with Hepatitis C Recombinant Mouse Monoclonal Antibody (rMMM33). Inset: PBS instead of primary antibody; secondary only negative control.
SDS-PAGE Analysis of Purified Hepatitis C virus (NS3) Recombinant Mouse Monoclonal Antibody (rMMM33). Confirmation of Purity and Integrity of Antibody.
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Non structural protein 3 (NS3) of hepatitis C virus (HCV) is a protein of 67 to 70 kD that contains at least three enzymatic activities; a protease, a serine protease and an RNA helicase. NS3 is produced as part of a polyprotein of about 3000 amino acids which is encoded by the viral genome. This polyprotein undergoes proteolytic processing by a combination of cellular and viral proteases, including those provided by NS3, to yield all of the mature viral proteins. The RNA helicase activity of NS3 is thought to be involved in viral replication.
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