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Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody [GFAP/8615R]

Additional information

Catalog Number: 2670-RBM33
Gene:

Host:

Reactivity:

,

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2670-RBM33-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2670-RBM33-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2670-RBM33-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody

SDS-PAGE Analysis of Purified GFAP Recombinant Rabbit Monoclonal Antibody (GFAP/8615R). Confirmation of Purity and Integrity of Antibody.

Recombinant GFAP (Astrocyte & Neural Stem Cell Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human brain stained with GFAP Recombinant Rabbit Monoclonal Antibody (GFAP/8615R). Inset: PBS instead of primary antibody; secondary only negative control.

GFAP Antibody in Western Blot (WB)

GFAP Antibody in Western Blot (WB)

Western Blot Analysis of human brain tissue lysate using GFAP Recombinant Rabbit Monoclonal Antibody (GFAP/8615R).

GFAP Antibody in Western Blot (WB)

GFAP Antibody in Western Blot (WB)

Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain and Guinea pig Brain tissue lysates using GFAP Recombinant Rabbit Monoclonal Antibody (GFAP/8615R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Guinea Pig, Human
Clone
GFAP/8615R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm
Gene Name
GFAP
Positive Control
T98G cells. Human brain or astrocytoma.
Immunogen
Recombinant fragment (around aa195-341) of the human GFAP protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2670
SwissProt
P14136
Chromosome Location
17q21.31
Mol. Weight of Antigen
49.88kDa
Alternate Names
Glial fibrillary acidic protein, ALXDRD

Summary

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

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