Contact Us

Recombinant FOXA1 / HNF3A Antibody [FOXA1/2230R]

Additional information

Catalog Number: 3169-RBM10
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

download data sheet
In Stock
Catalog Number Formulation Size Price
3169-RBM10-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3169-RBM10-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3169-RBM10-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant FOXA1 / HNF3A Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with FOXA1 Rabbit Recombinant Monoclonal Antibody (FOXA1/2230R).

Recombinant FOXA1 / HNF3A Antibody - Image 2

SDS-PAGE Analysis of Purified FOXA1 Rabbit Recombinant Monoclonal Antibody (FOXA1/2230R).

Recombinant FOXA1 / HNF3A Antibody - Image 3

Western Blot Analysis of HeLa cell lysate using FOXA1 Rabbit Recombinant Monoclonal Antibody (FOXA1/2230R).

Recombinant FOXA1 / HNF3A Antibody - Image 4

Flow Cytometric Analysis of PFA fixed MCF-7 cells using FOXA1 Rabbit Recombinant Monoclonal Antibody (FOXA1/2230R); followed by goat anti-rabbit IgG-CF488 (Blue); Goat anti-rabbit IgG-CF488 Is Control (Red).

Recombinant FOXA1 / HNF3A Antibody - Image 5

Immunofluorescence Analysis of PFA-fixed MCF-7 cells labeled with FOXA1 Rabbit Recombinant Monoclonal Antibody (FOXA1/2230R); followed by goat anti- rabbit IgG- CF488. Phalloiden stains the membrane(Red).

FOXA1 Antibody in SDS-PAGE

FOXA1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Hepatocyte nuclear factor 3-alpha Recombinant Rabbit Monoclonal Antibody (FOXA1/2230R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
FOXA1/2230R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
FOXA1
Positive Control
Bladder, Colon and Lung and Breast., HeLa, HepG-2 or MCF-7 cells. Liver, Pancreas, prostate
Immunogen
Recombinant fragment (around aa272-426) of the human FOXA1 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH16/1071).
7
  • Flow
  • IHC
  • WB

Recombinant Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-MSM4
Reactivity:

, , , ,

Gene:

Host:

Formalin-fixed, paraffin-embedded mouse heart stained with N-Cadherin Mouse Monoclonal Antibody (8C11).
3
  • Flow
  • IF
  • IHC
  • WB

N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM2
Reactivity:

,

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4443). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
5
  • IHC
  • WB

MICA Antibody

Additional information

Catalog Number: 100507436-MSM3
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human kidney stained with MICA Mouse Recombinant Monoclonal Antibody (MICA/4442). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.
5
  • IHC
  • WB

MICA Antibody

Additional information

Catalog Number: 100507436-MSM2
Reactivity:

, , , ,

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Cadherin 16 Mouse Monoclonal Antibody (CDH16/2448).
3
  • ELISA
  • IHC

Ksp-Cadherin / CDH16 (Renal Cell Marker) Antibody

Additional information

Catalog Number: 1014-MSM6
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Colon stained with Cadherin 17 / CDH17 Mouse Monoclonal Antibody (CDH17/2618).
4
  • Flow
  • IHC
  • WB

Cadherin 17 / LI Cadherin (Gastric Adenocarcinoma Marker) Antibody

Additional information

Catalog Number: 1015-MSM8
Reactivity:

Gene:

Host:

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Rabbit Recombinant Monoclonal Antibody (CDH16/1532R)
6
  • IHC
  • WB
  • Flow

Recombinant Ksp-Cadherin (Kidney-Specific Cadherin) / CDH16 Antibody

Additional information

Catalog Number: 1014-RBM2
Gene:

Host:

Reactivity:

Formalin-fixed, paraffin-embedded human heart stained with N-Cadherin Recombinant Mouse Monoclonal Antibody (rCDH2/1426).
2
  • IHC
  • WB

Recombinant N-Cadherin / Cadherin-2 / CD325 (NCAD) Antibody

Additional information

Catalog Number: 1000-MSM4
Gene:

Host:

Reactivity:

VIEW ALL ANTIBODIES

Other Information

Functions
  • Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). Proposed to play a role in translating the epigenetic signatures into cell type-specific enhancer-driven transcriptional programs. Its differential recruitment to chromatin is dependent on distribution of histone H3 methylated at 'Lys-5' (H3K4me2) in estrogen-regulated genes. Involved in the development of multiple endoderm-derived organ systems such as liver, pancreas, lung and prostate; FOXA1 and FOXA2 seem to have at least in part redundant roles (By similarity). Modulates the transcriptional activity of nuclear hormone receptors. Is involved in ESR1-mediated transcription; required for ESR1 binding to the NKX2-1 promoter in breast cancer cells; binds to the RPRM promoter and is required for the estrogen-induced repression of RPRM. Involved in regulation of apoptosis by inhibiting the expression of BCL2. Involved in cell cycle regulation by activating expression of CDKN1B, alone or in conjunction with BRCA1. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3169
SwissProt
P55317
Chromosome Location
14q21.1
Mol. Weight of Antigen
49.15kDa
Alternate Names
Hepatocyte nuclear factor 3-alpha, HNF3A|TCF3A

Summary

The transcription factor Forkhead-box A1 (FOXA1), also known as hepatocyte nuclear factor 3-alpha, is a member of the FOX class of transcription factors.HNF-1 (), and HNF-6 compose, in part, a homeoprotein family designated the hepatocyte nuclear factor family. The various HNF-1 isoforms regulate transcription of genes in the liver as well as in other tissues such as kidney, small intestine and thymus. FOXA1 is expressed in normal breast ductal epithelium and other epithelium in different organs, such as lung, pancreas, bladder, prostate, and colon. Recently, FOXA1 has been shown to be a major determinant of estrogen-ER activity and endocrine response in breast cancer cells. FOXA1 expression correlates with estrogen receptor (ER)-positivity, especially in luminal subtype A breast cancers, which is associated with favorable prognosis. FOXA1 is useful in the sub-classification of breast carcinomas.

Worry-Free Guarantee

Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.

Learn More

Need Technical Help?

Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.

CONTACT US

Follow Us on LinkedIn

NeoBiotechnologies develops and manufactures highly specific, validated monoclonal antibodies for cancer research and diagnostics.

Phone

Whatsapp

Quick Links

Information

© 2026 NeoBiotechnologies.  All Rights Reserved.

Terms and Conditions Privacy Policy
Contact Us