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Recombinant Ferritin, Light Chain (Node-Negative Breast Tumor Prognostic Marker) Antibody [FTL/3872R]

Additional information

Catalog Number: 2512-RBM9
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2512-RBM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2512-RBM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2512-RBM9-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Ferritin, Light Chain (Node-Negative Breast Tumor Prognostic Marker) Antibody

Formalin-fixed, paraffin-embedded human breast carcinoma stained with Ferritin, Light Chain Recombinant Rabbit Monoclonal Antibody (FTL/3872R).

Recombinant Ferritin, Light Chain (Node-Negative Breast Tumor Prognostic Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified Ferritin, Light Chain Recombinant Rabbit Monoclonal Antibody (FTL/3872R). Confirmation of Integrity and Purity of Antibody

Recombinant Ferritin, Light Chain (Node-Negative Breast Tumor Prognostic Marker) Antibody - Image 3

Western Blot Analysis of human kidney tissue lysate using Ferritin, Light Chain Recombinant Rabbit Monoclonal Antibody (FTL/3872R).

Ferritin Light Chain in Western Blot (WB)

Ferritin Light Chain in Western Blot (WB)

Western blot analysis of A-431 cell lysate using FTL Recombinant Rabbit Monoclonal Antibody (FTL/3872R).

Ferritin Light Chain in Western Blot (WB)

Ferritin Light Chain in Western Blot (WB)

Western blot analysis of Human Brain, Mouse Brain, Rat Brain, Human Lung, Mouse Lung, Rat Lung, Human Kidney, Mouse Kidney and Rat Kidney tissue lysates using Ferritin Light Chain Recombinant Rabbit Monoclonal Antibody (FTL/3872R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
FTL/3872R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Cytoplasmic vesicle, Autolysosome, Autophagosome
Gene Name
FTL
Positive Control
HeLa or 293T cells. Human kidney, HePG2, liver or testis., Pancreas or Human Brain, Lung
Immunogen
Recombinant fragment (around aa38-165) of the human Ferritin Light Chain protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney (By similarity). Delivery to lysosomes by the cargo receptor NCOA4 for autophagic degradation and release or iron (PubMed:24695223).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2512
SwissProt
P02792
Chromosome Location
19q13.33
Mol. Weight of Antigen
20.02kDa
Alternate Names
Ferritin light chain, FTL1|LFTD|NBIA3

Summary

Mammalian ferritins consist of 24 subunits made up of 2 types of polypeptide chains, ferritin heavy chain and ferritin light chain. Ferritin heavy chains catalyze the first step in iron storage, the oxidation of Fe (II), whereas ferritin light chains promote the nucleation of ferrihydrite, enabling storage of Fe (III). Light chain ferritin is involved in cataracts by at least two mechanisms, hereditary hyperferritinemia cataract syndrome, in which light chain ferritin is overexpressed, and oxidative stress, an important factor in the development of ageing-related cataracts.

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