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Recombinant Elastin (ELN) (Marker of Arterial Stiffness and Atherosclerosis) Antibody [MSVA-648R]

Additional information

Catalog Number: 2006-RBM6
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2006-RBM6-P0
Purified Ab with BSA and Azide
20ug
$259.00
2006-RBM6-P1
Purified Ab with BSA and Azide
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
ELN Antibody in Numerous Tissues (IHC)

ELN Antibody in Numerous Tissues (IHC)

Elastin Rabbit Recombinant Monoclonal Antibody (MSVA-648R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1:100-1:200 IHC Protocol Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MSVA-648R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Extracellular matrix, Extracellular space, Secreted
Gene Name
ELN
Positive Control
Colon: A moderate to strong elastin immunostaining should be seen of fibres in the lamina propria, around smooth muscle cells of the muscularis mucosae, and in blood vessels (subendothelial).
Immunogen
Recombinant full-length human Elastin protein
Expression System
HEK293
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Major structural protein of tissues such as aorta and nuchal ligament, which must expand rapidly and recover completely. Molecular determinant of the late arterial morphogenesis, stabilizing arterial structure by regulating proliferation and organization of vascular smooth muscle (By similarity).
Key References
  • Fazio, M.J., et al. Am. J. Hum. Genet. 48: 696-703.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2006
SwissProt
P15502
Chromosome Location
7q11.23
Mol. Weight of Antigen
70kDa
Alternate Names
Elastin, Tropoelastin, Elastin; ELN; SVAS; Tropoelastin; WBS; WS

Summary

Elastin is a polymeric protein and is the main component of the extracellular matrix of arteries.It is synthesized and secreted as a soluble, single-chain protein (tropoelastin), which undergoes a number of post-translational modifications prior to its organization into an elastic fiber in the extracellular space. Elastin performs a regulatory function during arterial development by controlling proliferation of smooth muscle and stabilizing arterial structure. It imparts elasticityto the connective tissue. During aging, the elasticity of connective tissue is reduced because of the cross-linking of collagenous fibers with elastin. The abnormal accumulation of elastic tissue in blood vessels is found in atherosclerosis and hypertension.

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