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Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody [KRT/1877R]

In Stock
Catalog Number Formulation Size Price
RBM37-1877-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
RBM37-1877-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
RBM37-1877-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Skin stained with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R).

Recombinant Cytokeratin, Multi (Epithelial Marker) Antibody - Image 2

Immunofluorescence Analysis of MeOH-fixed Human MCF-7 cells labeling CK with Multi-Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R)followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Cytokeratin Antibody in Western Blot (WB)

Cytokeratin Antibody in Western Blot (WB)

Western Blot Analysis of A431 cell lysate using Cytokeratin Recombinant Rabbit Monoclonal Antibody (KRT/1877R).

KRT6A Antibody in SDS-PAGE

KRT6A Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Keratin, type II cytoskeletal 6A Recombinant Rabbit Monoclonal Antibody (KRT/1877R). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI 6.0) subfamilies. This antibody recognizes acidic (Type I or LMW) and basic (Type II or HMW) cytokeratins, including 59kDa (CK4); 58kDa (CK5); 56kDa (CK6); 52kDa (CK8); 56.5kDa (CK10); 53kDa (CK13) and 45kDa (CK18). This is a broad-spectrum antibody, which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Gene Name
KRT6A
Positive Control
A431 cells, Colon carcinoma., Skin
Immunogen
Keratin-enriched preparation from cultured human epithelial cells
Alternate Names
Keratin, type II cytoskeletal 6A, CK-6C|CK-6E|CK6A|CK6C|CK6D|K6A|K6C|K6D|KRT6C|KRT6D|PC3

Database Links

Entrez Gene ID
3853
SwissProt
P02538

Additional Information

Clone
KRT/1877R
Chromosome Location
12q13.13
Mol. Weight of Antigen
60.04kDa
Expression System
HEK293 Cells
Purification
Protein A

Related Antibodies

View All KRT6A Antibodies

Functions

  • Epidermis-specific type I keratin involved in wound healing. Involved in the activation of follicular keratinocytes after wounding, while it does not play a major role in keratinocyte proliferation or migration. Participates in the regulation of epithelial migration by inhibiting the activity of SRC during wound repair.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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