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Recombinant Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) Antibody [rKRT10/844]

Additional information

Catalog Number: 3858-MSM9
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3858-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3858-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3858-MSM9-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin 10 (KRT10) (Suprabasal Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human Skin stained with Cytokeratin 10 Mouse Recombinant Monoclonal Antibody (rKRT10/844).

KRT10 Antibody in SDS-PAGE

KRT10 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Keratin, type I cytoskeletal 10 Recombinant Mouse Monoclonal Antibody (rKRT10/844). Confirmation of Purity and Integrity of Antibody.

Cytokeratin 10 Antibody in Western Blot (WB)

Cytokeratin 10 Antibody in Western Blot (WB)

Western blot analysis of Human Skin tissue lysate using Cytokeratin 10 Recombinant Mouse Monoclonal Antibody (rKRT10/844).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rKRT10/844
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell surface, Cytoplasm, Extracellular space, Secreted
Gene Name
KRT10
Positive Control
A431, HeLa, MCF7 cells. Esophagus or tonsil. Skin.
Immunogen
psoriasis patient skin extract of human origin
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity). Involved in the maintenance of cell layer development and keratin filament bundles in suprabasal cells of the epithelium (By similarity)., (Microbial infection) Acts as a mediator of S.aureus adherence to desquamated nasal epithelial cells via clfB, and hence may play a role in nasal colonization., (Microbial infection) Binds S.pneumoniae PsrP, mediating adherence of the bacteria to lung cell lines. Reduction of levels of KRT10 keratin decrease adherence, overexpression increases adherence. Neither protein has to be glycosylated for the interaction to occur.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3858
SwissProt
P13645
Chromosome Location
17q21.2
Mol. Weight of Antigen
58.83kDa
Alternate Names
Keratin, type I cytoskeletal 10, BCIE|BIE|CK10|EHK|EHK2|EHK2A|EHK2B|IHL|K10|KPP

Summary

This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.

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