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Recombinant Cytokeratin 1 (Suprabasal Keratinocyte Marker) Antibody [KRT1/8342R]

In Stock
Catalog Number Formulation Size Price
3848-RBM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3848-RBM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3848-RBM6-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Cytokeratin 1 (Suprabasal Keratinocyte Marker) Antibody

Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody (KRT1/8342R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant Cytokeratin 1 (Suprabasal Keratinocyte Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human skin stained with Cytokeratin 1 Recombinant Rabbit Monoclonal Antibody (KRT1/8342R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant Cytokeratin 1 (Suprabasal Keratinocyte Marker) Antibody - Image 3

SDS-PAGE Analysis Purified KRT1 Recombinant Rabbit Monoclonal Antibody (KRT1/8342R). Confirmation of Integrity and Purity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Keratins are a family of intermediate filament proteins that assemble into filaments through forming heterodimers of one type I keratin (keratins 9 to 23) and one type II keratin (keratins 1 to 8). Keratins demonstrate tissue and differentiation specific expression profiles. Cytokeratin 1 is a hetero-tetramer of two type I as well as two type II keratins, keratin-10 (KRT10). Keratin 1 is differentiation specific keratin that is one of the predominant keratins in suprabasal keratinocytes in stratified epithelia. Mutations in keratin 1 cause epidermolytic hyperkeratosis.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane, Cytoplasm
Gene Name
KRT1
Positive Control
Human skin or cervix.
Immunogen
Recombinant fragment corresponding to the c-terminal region of the human Cytokeratin 1 protein
Alternate Names
Keratin, type II cytoskeletal 1, AEI2|CK1|EHK|EHK1|EPPK|K1|KRT1A|NEPPK

Database Links

Entrez Gene ID
3848
SwissProt
P04264

Additional Information

Clone
KRT1/8342R
Chromosome Location
12q13.13
Mol. Weight of Antigen
66.04kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All KRT1 Antibodies

Functions

  • May regulate the activity of kinases such as PKC and SRC via binding to integrin beta-1 (ITB1) and the receptor of activated protein C kinase 1 (RACK1). In complex with C1QBP is a high affinity receptor for kininogen-1/HMWK.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
PARTNERSHIP OPPORTUNITIES

NeoBiotechnologies holds Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

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