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Recombinant Creatine Kinase-BB (CK-BB) Antibody [CKBB/8843R]

Additional information

Catalog Number: 1152-RBM22
Gene:

Host:

Reactivity:

, , , ,

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
1152-RBM22-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
1152-RBM22-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
1152-RBM22-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Creatine Kinase-BB (CK-BB) Antibody

Formalin-fixed, paraffin-embedded human brain stained with CKBB Recombinant Rabbit Monoclonal Antibody (CKBB/8843R). Inset: PBS instead of primary antibody; secondary only negative control.

Recombinant Creatine Kinase-BB (CK-BB) Antibody - Image 2

SDS-PAGE Analysis of Purified CKBB Recombinant Rabbit Monoclonal Antibody (CKBB/8843R). Confirmation of Purity and Integrity of Antibody.

CKBB Antibody in Western blot (WB)

CKBB Antibody in Western blot (WB)

Western Blot Analysis of HEK293T, Y79 cell lysate using CKBB Recombinant Rabbit Monoclonal Antibody (CKBB/8843R).

CKBB Antibody in Western Blot (WB)

CKBB Antibody in Western Blot (WB)

Western blot analysis of Y79 cell lysate using CKBB Rabbit Recombinant Monoclonal Antibody (CKBB/8843R).

CKBB Antibody in Western Blot (WB)

CKBB Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach and Guinea pig Stomach tissue lysates using CKBB Rabbit Recombinant Monoclonal Antibody (CKBB/8843R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Guinea Pig, Hamster, Human, Mouse, Rat
Clone
CKBB/8843R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane, Cytoplasm, Cytosol, Mitochondrion
Gene Name
CKB
Positive Control
Y79 or HEK293T, Y79 cells. Human cerebellum. Human Brain, Mouse Brain, Rat Brain, Hamster Brain, Guinea pig Brain, Human Stomach, Mouse Stomach, Rat Stomach, Hamster Stomach and Guinea pig Stomach
Immunogen
Recombinant fragment (around aa261-376) of the human CKBB protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate) (PubMed:8186255). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa (Probable). Acts as a key regulator of adaptive thermogenesis as part of the futile creatine cycle: localizes to the mitochondria of thermogenic fat cells and acts by mediating phosphorylation of creatine to initiate a futile cycle of creatine phosphorylation and dephosphorylation (By similarity). During the futile creatine cycle, creatine and N-phosphocreatine are in a futile cycle, which dissipates the high energy charge of N-phosphocreatine as heat without performing any mechanical or chemical work (By similarity).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
1152
SwissProt
P12277
Chromosome Location
14q32.33
Mol. Weight of Antigen
42.64kDa
Alternate Names
Creatine kinase B-type, B-CK|BCK|CKBB|CPK-B|HEL-211|HEL-S-29

Summary

Creatine kinases (CK) are a large family of isoenzymes that regulate levels of ATP in subcellular compartments, where they provide ATP at sites of fluctuating energy demand by the transfer of phosphates between creatine and adenine nucleotides. CKs provide the energy of phosphate hydrolysis necessary to drive the normal function of many cellular systems. In cells, the cytosolic CK enzymes consist of two subunits, which can be either B (brain type) or M (muscle type). There are three different isoenzymes: CKMM, CKBB and CKMB. This MAb recognizes the CKBB isoenzyme and does not react with the B subunit in CKMB.

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