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Recombinant CEACAM6 / CD66c / CEA (Pancreatic Adenocarcinoma Biomarker) Antibody [CEACAM6/13371R]

Additional information

Catalog Number: 4680-RBM3
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4680-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
4680-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4680-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CEACAM6 Antibody in Immunohistochemistry (IHC (P))

CEACAM6 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human colon carcinoma stained with CEACAM6 / CD66c Recombinant Rabbit Monoclonal Antibody (CEACAM6/13371R). Inset: PBS instead of primary antibody; secondary only negative control.

CEACAM6 Antibody in SDS-PAGE

CEACAM6 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CEACAM6 Recombinant Rabbit Monoclonal Antibody (CEACAM6/13371R). Confirmation of Purity and Integrity of Antibody.

CEACAM6 Antibody in Western Blot (WB)

CEACAM6 Antibody in Western Blot (WB)

Western Blot Analysis of HT-29 and AsPC-1 cell lysates using CEACAM6 Recombinant Rabbit Monoclonal Antibody (CEACAM6/13371R).

  • ELISA
  • Flow
  • Functional Studies
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
ELISA, Flow Cytometry, Functional Studies, Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CEACAM6/13371R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Cell surface
Gene Name
CEACAM6
Positive Control
Human appendix, colon or pancreatic cancer. HT-29 or AsPC-1.
Immunogen
Recombinant CEACAM6 protein
Expression System
CHO
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Reactivity:

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Cell surface glycoprotein that plays a role in cell adhesion and tumor progression (PubMed:10910050, PubMed:11590190, PubMed:1378450, PubMed:16204051, PubMed:2022629, PubMed:2803308, PubMed:8776764). Intercellular adhesion occurs in a calcium- and fibronectin-independent manner (PubMed:16204051, PubMed:2022629). Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8 (PubMed:11590190, PubMed:16204051, PubMed:2022629, PubMed:2803308, PubMed:8776764). Heterophilic interaction with CEACAM8 occurs in activated neutrophils (PubMed:8776764). Plays a role in neutrophil adhesion to cytokine-activated endothelial cells (PubMed:1378450). Plays a role in cell migration and cell adhesion to endothelial cells (PubMed:16204051).
Key References
  • Kolla, Venkatadri et al. “Carcinoembryonic cell adhesion molecule 6 in human lung: regulated expression of a multifunctional type II cell protein.” American journal of physiology. Lung cellular and molecular physiology vol. 296,6 (2009): L1019-30. doi:10.1152/ajplung.90596.2008
  • Wu, Guanhua et al. “The emerging roles of CEACAM6 in human cancer (Review).” International journal of oncology vol. 64,3 (2024): 27. doi:10.3892/ijo.2024.5615
  • Pinkert, Jessica et al. “T cell-mediated elimination of cancer cells by blocking CEACAM6-CEACAM1 interaction.” Oncoimmunology vol. 11,1 2008110. 30 Dec. 2021, doi:10.1080/2162402X.2021.2008110
  • Rinchai, Darawan, and Damien Chaussabel. “Assessing the potential relevance of CEACAM6 as a blood transcriptional biomarker.” F1000Research vol. 11 1294. 4 Apr. 2024, doi:10.12688/f1000research.126721.2
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4680
SwissProt
P40199
Chromosome Location
19q13.2
Mol. Weight of Antigen
90kDa
Alternate Names
Cell adhesion molecule CEACAM6, Carcinoembryonic antigen-related cell adhesion molecule 6 (CEA cell adhesion molecule 6 ), Non-specific crossreacting antigen, Normal cross-reacting antigen, CEACAM6, NCA, CD66c

Summary

The CD66 (also designated carcinoembryonic antigen, CEA, biliary glycoprotein I, BGP-1, CEACAM) immunoglobulin superfamily of genes encode cell adhesion proteins, which are expressed at higher levels in tumorous tissues than in normal tissues. CD66 mRNA is strongly expressed in primary colon tumors and, to a lesser extent, in normal colonic tissue. The human CD66 gene family is a diverse set of glycoproteins of epithelial and hematopoietic lineage that comprises 29 genes, which map to chromosome position 19q13.1-q13.2. CD66A, CD66B, CD66C, CD66D, CD66E and CD66F are the best characterized CD66 antigens, and CD66A-D expression upregulates on the surface of granulocytes upon stimulation. Certain CD66 isoforms mediate homotypic and heterotypic intercellular adhesion events independently of cell type.

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