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Recombinant CD70 / TNFSF7 (Activated T- & B-Lymphocyte Marker) Antibody [CD70/12949R]

In Stock
Catalog Number Formulation Size Price
970-RBM26-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
970-RBM26-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
970-RBM26-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CD70 Antibody in SDS-PAGE

CD70 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD70 Recombinant Rabbit Monoclonal Antibody (CD70/12949R). Confirmation of Purity and Integrity of Antibody.

CD70 Antibody in Western Blot (WB)

CD70 Antibody in Western Blot (WB)

Western Blot Analysis of HeLa and Jurkat cell lysates using CD70 Recombinant Rabbit Monoclonal Antibody (CD70/12949R).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

It recognizes a protein of 30kDa, identified as CD70. It is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T- and B-lymphocytes. It induces proliferation of co-stimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin synthesis.

Product Properties & Targets

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cell membrane
Gene Name
CD70
Positive Control
Raji cells. Lymph node or tonsil. HeLa or Jurkat.
Immunogen
Recombinant fragment (around aa176-193) of the human CD70 protein (exact sequence is proprietary)
Alternate Names
CD70 antigen, CD27-L|CD27L|CD27LG|LPFS3|TNFSF7|TNLG8A

Database Links

Entrez Gene ID
970
SwissProt
P32970

Additional Information

Clone
CD70/12949R
Chromosome Location
19p13.3
Mol. Weight of Antigen
21.12kDa
Expression System
CHO Cells
Purification
Protein A

Related Antibodies

View All CD70 Antibodies

Functions

  • Expressed at the plasma membrane of B cells, it is the ligand of the CD27 receptor which is specifically expressed at the surface of T cells (PubMed:28011863, PubMed:28011864, PubMed:8387892). The CD70-CD27 signaling pathway mediates antigen-specific T cell activation and expansion which in turn provides immune surveillance of B cells (PubMed:28011863, PubMed:28011864).

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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