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Recombinant CD6 (Negative Marker of T-regulatory Cells) Antibody [C6/2884R]

Additional information

Catalog Number: 923-RBM5
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
923-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
923-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
923-RBM5-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD6 (Negative Marker of T-regulatory Cells) Antibody

Formalin-fixed, paraffin-embedded human Tonsil stained with CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R).

Recombinant CD6 (Negative Marker of T-regulatory Cells) Antibody - Image 2

Flow Cytometric Analysis of MOLT-4 cells. CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype control (Red).

Recombinant CD6 (Negative Marker of T-regulatory Cells) Antibody - Image 3

Immunofluorescence staining of MOLT-4 cells using CD6 Rabbit Recombinant Monoclonal Antibody (C6/2884R) followed by goat anti-rabbit IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

CD6 Antibody in SDS-PAGE

CD6 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified T-cell differentiation antigen CD6 Recombinant Rabbit Monoclonal Antibody (C6/2884R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Flow
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Clone
C6/2884R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell membrane, Secreted
Gene Name
CD6
Positive Control
CCRF-CEM, Jurkat cells, Molt-4, Tonsil.
Immunogen
Recombinant full-length human CD6 protein
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Cell adhesion molecule that mediates cell-cell contacts and regulates T-cell responses via its interaction with ALCAM/CD166 (PubMed:15048703, PubMed:15294938, PubMed:16352806, PubMed:16914752, PubMed:24584089, PubMed:24945728). Contributes to signaling cascades triggered by activation of the TCR/CD3 complex (PubMed:24584089). Functions as a costimulatory molecule; promotes T-cell activation and proliferation (PubMed:15294938, PubMed:16352806, PubMed:16914752). Contributes to the formation and maturation of the immunological synapse (PubMed:15294938, PubMed:16352806). Functions as a calcium-dependent pattern receptor that binds and aggregates both Gram-positive and Gram-negative bacteria. Binds both lipopolysaccharide (LPS) from Gram-negative bacteria and lipoteichoic acid from Gram-positive bacteria (PubMed:17601777). LPS binding leads to the activation of signaling cascades and down-stream MAP kinases (PubMed:17601777). Mediates activation of the inflammatory response and the secretion of pro-inflammatory cytokines in response to LPS (PubMed:17601777).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
923
SwissProt
P30203
Chromosome Location
11q12.2
Mol. Weight of Antigen
71.8kDa
Alternate Names
T-cell differentiation antigen CD6, TP120

Summary

CD6 is a type I transmembrane glycoprotein that contains a 24-amino acid signal sequence, three extracellular scavenger receptor cysteine-rich (SRCR) domains, a membrane-spanning domain and a 44-amino acid cytoplasmic domain. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T cell activation. CD6 is present on mature thymocytes, peripheral T cells and a subset of B cells. Antibodies to CD6 are used to deplete T cells from bone marrow transplants to prevent graft versus host disease.

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