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Recombinant CD44 / HCAM (Cancer Stem Cell Marker) Antibody [rCD44v9/1459]

Additional information

Catalog Number: 960-MSM13
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
960-MSM13-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
960-MSM13-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
960-MSM13-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD44 / HCAM (Cancer Stem Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Cervical Carcinoma stained with CD44v9 Mouse Recombinant Monoclonal Antibody (rCD44v9/1459).

CD44 Antibody in SDS-PAGE

CD44 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD44 antigen Recombinant Mouse Monoclonal Antibody (rCD44v9/1459). Confirmation of Purity and Integrity of Antibody.

CD44v9 Antibody in Western Blot (WB)

CD44v9 Antibody in Western Blot (WB)

Western Blot Analysis of A431 cell lysate using CD44 Recombinant Mouse Monoclonal Antibody (rCD44v9/1459).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
rCD44v9/1459
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell membrane, Cell projection, Microvillus, Secreted
Gene Name
CD44
Positive Control
Cervix or Tongue Squamous Cell Carcinoma. A431.
Immunogen
Fusion protein of human CD44
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Cell-surface receptor that plays a role in cell-cell interactions, cell adhesion and migration, helping them to sense and respond to changes in the tissue microenvironment (PubMed:16541107, PubMed:19703720, PubMed:22726066). Participates thereby in a wide variety of cellular functions including the activation, recirculation and homing of T-lymphocytes, hematopoiesis, inflammation and response to bacterial infection (PubMed:7528188). Engages, through its ectodomain, extracellular matrix components such as hyaluronan/HA, collagen, growth factors, cytokines or proteases and serves as a platform for signal transduction by assembling, via its cytoplasmic domain, protein complexes containing receptor kinases and membrane proteases (PubMed:18757307, PubMed:23589287). Such effectors include PKN2, the RhoGTPases RAC1 and RHOA, Rho-kinases and phospholipase C that coordinate signaling pathways promoting calcium mobilization and actin-mediated cytoskeleton reorganization essential for cell migration and adhesion (PubMed:15123640).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
960
SwissProt
P16070
Chromosome Location
11p13
Mol. Weight of Antigen
81.54kDa
Alternate Names
CD44 antigen, CDW44|CSPG8|ECM-III|ECMR-III|H-CAM|HCELL|HUTCH-1|HUTCH-I|Hermes-1|LHR|MC56|MDU2|MDU3|MIC4|Pgp1

Summary

This antibody recognizes an epitope encoded by exon v4 on the variant portion of human CD44. The CD44 molecule belongs to a family of cellular adhesion molecules found on a wide range of normal and malignant cells in epithelial, mesothelial and hemopoiesis tissues. CD44 is a single gene with 20 exons, of which 10 are normally expressed to encode the basic CD44 (H-CAM) molecule. The additional 10 exons (v1 to v10) are only expressed by alternative splicing of the nuclear RNA.The expression of specific cell adhesion molecule CD44 splice variants has been reported to be associated with metastasis in certain human malignancies.

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