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Recombinant CD171 / NCAM-L1 (Axonal Marker) Antibody [L1CAM/9146R]

Additional information

Catalog Number: 3897-RBM7
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
3897-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
3897-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
3897-RBM7-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD171 / NCAM-L1 (Axonal Marker) Antibody

SDS-PAGE Analysis of Purified CD171 Recombinant Rabbit Monoclonal Antibody (L1CAM/9146R). Confirmation of Integrity and Purity of Antibody.

Recombinant CD171 / NCAM-L1 (Axonal Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human brain stained with CD171 Recombinant Rabbit Monoclonal Antibody (L1CAM/9146R). Inset: PBS instead of primary antibody; secondary only negative control.

CD171 Antibody in Western Blot (WB)

CD171 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain lysate using CD171 Rabbit Recombinant Monoclonal Antibody (L1CAM/9146R)

  • IHC
  • WB
  • Flow
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Clone
L1CAM/9146R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Axon, Cell membrane, Cell projection, Dendrite, Growth cone
Gene Name
L1CAM
Positive Control
Human brain total lysate (WB). PC12 and Neuro-2A (ICC).
Immunogen
Recombinant fragment (around aa1-1257) of the human CD171 protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
3897
SwissProt
P32004
Chromosome Location
Xq28
Mol. Weight of Antigen
140.0kDa
Alternate Names
Neural cell adhesion molecule L1, CAML1|CD171|HSAS|HSAS1|HYCX|MASA|MIC5|N-CAM-L1|N-CAML1|NCAM-L1|S10|SPG1

Summary

L1 (L1 cell adhesion molecule) also known as CAML1, CD171, and HSAS is an adhesion molecule which plays an important role in the nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). This mouse monoclonal antibody recognizes the sequence immediately NH2-terminal to the tyrosine-based motif and binds L1 only when Y1176 is dephosphorylated. Phosphorylation of either T1172 or Y1176 strongly inhibits binding of 74-5H7 to the L1 cytoplasmic domain.

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