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Recombinant CD162 (Selectin P Ligand) Antibody [PSGL1/8192R]

Additional information

Catalog Number: 6404-RBM5
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6404-RBM5-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6404-RBM5-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6404-RBM5-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant CD162 (Selectin P Ligand) Antibody

Formalin-fixed, paraffin-embedded human tonsil stained with CD162 Recombinant Rabbit Monoclonal Antibody (PSGL1/8192R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant CD162 (Selectin P Ligand) Antibody - Image 2

Formalin-fixed, paraffin-embedded human spleen stained with CD162 Recombinant Rabbit Monoclonal Antibody (PSGL1/8192R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant CD162 (Selectin P Ligand) Antibody - Image 3

IHC analysis of formalin-fixed, paraffin-embedded human brain. Negative tissue control using PSGL1/8192R at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

CD162 Antibody in SDS-PAGE

CD162 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CD162 Recombinant Rabbit Monoclonal Antibody (PSGL1/8192R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Flow
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot, Flow
Species Reactivity
Human
Clone
PSGL1/8192R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Membrane
Gene Name
SELPLG
Positive Control
Human tonsil or spleen.
Immunogen
Recombinant full-length human CD162 protein
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • A SLe(x)-type proteoglycan, which through high affinity, calcium-dependent interactions with E-, P- and L-selectins, mediates rapid rolling of leukocytes over vascular surfaces during the initial steps in inflammation. Critical for the initial leukocyte capture., (Microbial infection) Acts as a receptor for enterovirus 71.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6404
SwissProt
Q14242
Chromosome Location
12q24.11
Mol. Weight of Antigen
43.2kDa
Alternate Names
P-selectin glycoprotein ligand 1, CD162|CLA|PSGL-1|PSGL1

Summary

CD162 glycoprotein functions as a high affinity counter-receptor for the cell adhesion molecules P-, E- and L- selectin expressed on myeloid cells and stimulated T lymphocytes. As such, this protein plays a critical role in leukocyte trafficking during inflammation by tethering of leukocytes to activated platelets or endothelia expressing selectins. This protein requires two post-translational modifications, tyrosine sulfation and the addition of the sialyl Lewis x tetrasaccharide (sLex) to its O-linked glycans, for its high-affinity binding activity. Aberrant expression of this gene and polymorphisms in this gene are associated with defects in the innate and adaptive immune response. �

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