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Recombinant CD15 / FUT4 (Reed-Sternberg Cell Marker) Antibody [FUT4/1478R]

Additional information

Catalog Number: 2526-RBM8
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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Recombinant CD15 / FUT4 (Reed-Sternberg Cell Marker) Antibody

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R).

Recombinant CD15 / FUT4 (Reed-Sternberg Cell Marker) Antibody - Image 2

Immunofluorescence staining of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

Recombinant CD15 / FUT4 (Reed-Sternberg Cell Marker) Antibody - Image 3

Flow Cytometric Analysis of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

  • IHC
  • WB
In Stock
Catalog Number Formulation Availability Size Price
2526-RBM8-P0
Purified Ab with BSA and Azide at 200ug/ml
Trial Size
In Stock
20ug
$259.00
Trial Size Available In-Stock
2526-RBM8-P1
Purified Ab with BSA and Azide at 200ug/ml
In Stock
100ug
$559.00
In-Stock
2526-RBM8-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
Carrier-Free
In Stock
100ug
$559.00
Carrier-Free Available In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
FUT4/1478R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Golgi apparatus, Golgi stack membrane
Gene Name
FUT4
Positive Control
Reed-Sternberg's cells in Hodgkin's lymphoma., U937 cells
Immunogen
U937 histiocytic cell line
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins (PubMed:1702034, PubMed:1716630, PubMed:29593094). Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins (PubMed:1716630, PubMed:29593094). Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis (By similarity). In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE (PubMed:11278338, PubMed:1716630)., Does not generate Lewis X antigens.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2526
SwissProt
P22083
Chromosome Location
11q21
Mol. Weight of Antigen
59.08kDa
Alternate Names
Alpha-(1,3)-fucosyltransferase 4, CD15|ELFT|FCT3A|FUC-TIV|FUTIV|LeX|SSEA-1

Summary

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

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