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Recombinant Calponin-1 (Smooth Muscle Marker) Antibody [MSVA-455R]

In Stock
Catalog Number Formulation Size Price
1264-RBM16-P0
Purified Ab with BSA and Azide
20ug
$324.50
1264-RBM16-P1
Purified Ab with BSA and Azide
100ug
$599.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
CNN1 Antibody in Numerous Tissues (IHC)

CNN1 Antibody in Numerous Tissues (IHC)

Calponin-1 Rabbit Recombinant Monoclonal Antibody (MSVA-455R) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Calponin a 33 kDa polypeptide, is a cytoskeleton-associated actin-binding protein that also interacts tropomyosin and calmodulin. Calponin antibody has been found to be useful as a marker for myoepithelial and basal lamina in differentiating microinvasive from in situ ductal carcinomas of the breast. Calponin antibody may also have applications in malignant myoepithelium and pleomorphic adenoma of salivary gland as well as a useful marker for fine needle aspirates of papillary breast lesions. The antibody has been found in tissue extracts from smooth muscle, but not in fibroblast extracts.

Product Properties & Targets

Antibody Type
Rabbit Monoclonals, Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular, Mesenchymal Stem Cell Differentiation
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm
Gene Name
CNN1
Positive Control
Colon: A moderate to strong Calponin 1 staining should be seen in the muscularis mucosae, muscularis propria and in the walls of larger blood vessels.
Immunogen
Synthetic peptide corresponding to residues on the C-terminus of human Calponin was used as an immunogen
Alternate Names
Calponin-1, Basic calponin, Calponin H1, smooth muscle, Calponin 1 basic smooth muscle; Calponin H1 smooth muscle; Calponin-1; CNN1; Cnn1; Sm Calp; SMCC

Database Links

Entrez Gene ID
1264
SwissProt
P51911

Additional Information

Clone
MSVA-455R
Chromosome Location
19p13.2
Mol. Weight of Antigen
33kDa

Related Antibodies

View All CNN1 Antibodies

Functions

  • Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity (By similarity).

Key References

  • Tang, D.C., et al. 1996. Structure-function relations of smooth muscle Calponin. The critical role of Serine 175. J. Biol. Chem. 271: 8605-8611.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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