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Recombinant BRG1 / SMARCA4 Antibody [BRG1/8805R]

Additional information

Catalog Number: 6597-RBM3
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
6597-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
6597-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
6597-RBM3-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant BRG1 / SMARCA4 Antibody

Formalin-fixed, paraffin-embedded human colon stained with BRG1 / Recombinant Rabbit Monoclonal Antibody (BRG1/8805R). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Recombinant BRG1 / SMARCA4 Antibody - Image 2

SDS-PAGE Analysis of Purified BRG1 / Recombinant Rabbit Monoclonal Antibody (BRG1/8805R). Confirmation of Purity and Integrity of Antibody.

BRG1 Antibody in Western Blot (WB)

BRG1 Antibody in Western Blot (WB)

Western blot analyses of HeLa, Jurkat and K-562 cell lysates using BRG1 Recombinant Rabbit Monoclonal Antibody (BRG1/8805R).

BRG1 / SMARCA4 Antibody in Human Protein Array (HuProt) Analysis

BRG1 / SMARCA4 Antibody in Human Protein Array (HuProt) Analysis

Analysis of HuProt™ Protein Array (21,000+ full-length human proteins) with BRG1 / SMARCA4 Recombinant Rabbit Monoclonal Antibody (BRG1/8805R). Z-score: Signal strength (in SD above the mean) of antibody binding to each protein. S-score: Difference in Z-scores between the top target and the next best hit, indicating relative specificity. An antibody is considered specific if S ≥ 2.5. Example: Binding to protein X (Z = 43) vs protein Y (Z = 14) → S = 29.

BRG1 Antibody Validation in Western Blot on Knockdown(KD) cells.

BRG1 Antibody Validation in Western Blot on Knockdown(KD) cells.

Western Blot validation of anti-BRG1 antibody. BRG1 protein expression was compared in wild-type (WT) and BRG1 shRNA Knockdown (KD) HeLa cells (30ug of total protein). Loading control is shown below.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
BRG1/8805R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
SMARCA4
Positive Control
Jurkat, K-562, HeLa or HEK-293T cells. Ubiquitous nuclear expression.
Immunogen
Recombinant fragment (around aa249-268) of the human BRG1 protein (exact sequence is proprietary)
Expression System
CHO
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • ATPase involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner (PubMed:15075294, PubMed:29374058, PubMed:30339381, PubMed:32459350). Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP (By similarity). Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1 (PubMed:20418909). Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2 (By similarity). Binds to RNA in a promiscuous manner (By similarity). In brown adipose tissue, involved in the regulation of thermogenic genes expression (By similarity).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
6597
SwissProt
P51532
Chromosome Location
19p13.2
Mol. Weight of Antigen
184.64kDa
Alternate Names
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4, BAF190|BAF190A|BRG1|CSS4|MRD16|OTSC12|RTPS2|SNF2|SNF2-beta|SNF2L4|SNF2LB|SWI2|hSNF2b

Summary

The SWI-SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF2α) and Brg-1 (also designated SNF2β) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/ SNF, including the NURF (nucleosome remodeling factor).

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