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Recombinant Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody [B2M/1857R]

Additional information

Catalog Number: 567-RBM7
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
567-RBM7-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
567-RBM7-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
567-RBM7-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody

Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with B2M Rabbit Recombinant Monoclonal Antibody (B2M/1857R).

Recombinant Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 2

SDS-PAGE Analysis PurifiedB2M Rabbit Recombinant Monoclonal Antibody (B2M/1857R). Confirmation of Purity and Integrity of Antibody.

Recombinant Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 3

Flow Cytometric Analysis of human HeLa cells using Beta-2-Microglobulin Rabbit Recombinant Monoclonal Antibody (B2M/1857R) followed by goat anti-mouse IgG-CF488 (Blue); Isotype control (Red).

Recombinant Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 4

Western Blot Analysis of THP-1 and Raji cell lysate using B2M Rabbit Recombinant Monoclonal Antibody (B2M/1857R).

Recombinant Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 5

Immunofluorescence Analysis of PFA fixed HeLa cells labeling with Beta-2-Microglobulin Rabbit Recombinant Monoclonal Antibody (B2M/1857R); followed by goat anti-rabbit IgG-CF488 (Green).

Recombinant Beta-2 Microglobulin (Renal Failure & Tumor Marker) Antibody - Image 6

Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain,Human Lungs, Mouse Lungs and Rat Lungs tissue lysates using Beta-2 Microglobulin Rabbit Recombinant Monoclonal Antibody (B2M/1857R).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
B2M/1857R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cell surface, Secreted
Gene Name
B2M
Positive Control
Melanomas and Lymphoma. Carcinoma of Stomach. THP-1 or Raji.
Immunogen
Recombinant full-length human Beta-2 Microglobulin (B2M) protein
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
567
SwissProt
P61769
Chromosome Location
15q21.1
Mol. Weight of Antigen
13.71kDa
Alternate Names
Beta-2-microglobulin, AMYLD6|IMD43|MHC1D4

Summary

Recognizes a protein of 12kDa, identified as beta-2 microglobulin. Major histocompatibility complex (MHC) class 1 molecules bind to antigens for presentation on the surface of cells. The proteasome is responsible for producing these antigens from the components of foreign pathogens. MHC class 1 molecules consist of an alphaheavy chain that contains three subdomains (alpha1, alpha2, alpha3) and a non-covalent associating light chain, known as beta-2-Microglobulin. Beta-2-Microglobulin associates with the alpha3 subdomain of the alphaheavy chain and forms an immunoglobulin domain-like structure that mediates proper folding and expression of MHC class 1 molecules. The alpha1 and alpha2 domains of the alphaheavy chain form the peptide antigen-binding cleft. Mutations in the beta-2-Microglobulin gene can enhance the progression of malignant melanoma phenotypes.

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