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Recombinant B3GAT1 Antibody [rNK-1]

In Stock
Catalog Number Formulation Size Price
27087-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
27087-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
27087-MSM9-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
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Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

Product Properties & Targets

Antibody Type
Recombinant Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgM / Kappa
Cellular Localization
Endoplasmic reticulum membrane, Golgi apparatus membrane, Secreted
Gene Name
B3GAT1
Positive Control
Mainly expressed in the brain
Immunogen
Recombinant human B3GAT1 protein
Alternate Names
Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1, CD57|GLCATP|GLCUATP|HNK1|LEU7|NK-1|NK1

Database Links

Entrez Gene ID
27087
SwissProt
Q9P2W7

Additional Information

Clone
rNK-1
Chromosome Location
11q25
Mol. Weight of Antigen
38.26kDa
Purification
Protein L

Related Antibodies

View All B3GAT1 Antibodies

Functions

  • Involved in the biosynthesis of L2/HNK-1 carbohydrate epitope on glycoproteins. Can also play a role in glycosaminoglycan biosynthesis. Substrates include asialo-orosomucoid (ASOR), asialo-fetuin, and asialo-neural cell adhesion molecule. Requires sphingomyelin for activity: stearoyl-sphingomyelin was the most effective, followed by palmitoyl-sphingomyelin and lignoceroyl-sphingomyelin. Activity was demonstrated only for sphingomyelin with a saturated fatty acid and not for that with an unsaturated fatty acid, regardless of the length of the acyl group.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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