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Recombinant Androgen Receptor (Marker of Androgen Dependence) Antibody [DHTR.V7/9394R]

Additional information

Catalog Number: 367-RBM22
Gene:

Host:

Reactivity:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
367-RBM22-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
367-RBM22-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
367-RBM22-P1ABX
Purified Ab WITHOUT BSA or Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant Androgen Receptor (Marker of Androgen Dependence) Antibody

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with AR Recombinant Rabbit Monoclonal Antibody (DHTR.V7/9394R). HIER: Tris/EDTA, pH9.0, 95°C/45min. 2°CAb: HRP-Poly:30min. DAB:5min.

Recombinant Androgen Receptor (Marker of Androgen Dependence) Antibody - Image 2

SDS-PAGE Analysis of Purified AR Recombinant Rabbit Monoclonal Antibody (DHTR.V7/9394R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
DHTR.V7/9394R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm, Nucleus
Gene Name
AR
Positive Control
Breast|Human testis|prostate or prostate carcinoma.
Immunogen
Recombinant fragment (around aa629-644) of the human Androgen Receptor V7 protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein A
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in a mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Steroid hormone receptors are ligand-activated transcription factors that regulate eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues (PubMed:19022849). Transcription factor activity is modulated by bound coactivator and corepressor proteins like ZBTB7A that recruits NCOR1 and NCOR2 to the androgen response elements/ARE on target genes, negatively regulating androgen receptor signaling and androgen-induced cell proliferation (PubMed:20812024). Transcription activation is also down-regulated by NR0B2. Activated, but not phosphorylated, by HIPK3 and ZIPK/DAPK3., Lacks the C-terminal ligand-binding domain and may therefore constitutively activate the transcription of a specific set of genes independently of steroid hormones.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
367
SwissProt
P10275
Chromosome Location
Xq12
Mol. Weight of Antigen
99.19kDa
Alternate Names
Androgen receptor, AIS|AR8|DHTR|HUMARA|HYSP1|KD|NR3C4|SBMA|SMAX1|TFM

Summary

AR V7 belongs to the nuclear hormone receptor family. It contains the nuclear receptor DNA binding domain. Steroid hormone receptors are ligand-activated transcription factors that regulate the expression of eukaryotic genes affecting cell proliferation and differentiation in target tissues. The activity of transcription factors is regulated by binding co-activators and co-repressors. This target is a splice variant of the androgen receptor that lacks the C-terminal androgen-binding site. AR-V7 plays an important role in the occurrence, development and drug resistance of prostate cancer.

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