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Rb1 (Tumor Suppressor Protein) Antibody [RB1/1754]

Additional information

Catalog Number: 5925-MSM2
Reactivity:

,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
5925-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
5925-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
5925-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Rb1 (Tumor Suppressor Protein) Antibody

Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).

Rb1 (Tumor Suppressor Protein) Antibody - Image 2

Formalin-fixed, paraffin-embedded human Colon stained with Retinoblastoma (Rb1) Monoclonal Antibody (RB1/1754).

RB1 Antibody in SDS-PAGE

RB1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Retinoblastoma-associated protein Mouse Monoclonal Antibody (RB1/1754). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human, Mouse
Clone
RB1/1754
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Nucleus
Gene Name
RB1
Positive Control
A431 or BT-20 cells. Breast or Colon carcinoma., HeLa
Immunogen
Recombinant human Rb1 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Tumor suppressor that is a key regulator of the G1/S transition of the cell cycle (PubMed:10499802). The hypophosphorylated form binds transcription regulators of the E2F family, preventing transcription of E2F-responsive genes (PubMed:10499802). Both physically blocks E2Fs transactivating domain and recruits chromatin-modifying enzymes that actively repress transcription (PubMed:10499802). Cyclin and CDK-dependent phosphorylation of RB1 induces its dissociation from E2Fs, thereby activating transcription of E2F responsive genes and triggering entry into S phase (PubMed:10499802). RB1 also promotes the G0-G1 transition upon phosphorylation and activation by CDK3/cyclin-C (PubMed:15084261). Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV39H1, KMT5B and KMT5C, leading to epigenetic transcriptional repression. Controls histone H4 'Lys-20' trimethylation. Inhibits the intrinsic kinase activity of TAF1. Mediates transcriptional repression by SMARCA4/BRG1 by recruiting a histone deacetylase (HDAC) complex to the c-FOS promoter. In resting neurons, transcription of the c-FOS promoter is inhibited by BRG1-dependent recruitment of a phospho-RB1-HDAC1 repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex (By similarity).
  • (Microbial infection) In case of viral infections, interactions with SV40 large T antigen, HPV E7 protein or adenovirus E1A protein induce the disassembly of RB1-E2F1 complex thereby disrupting RB1's activity.
Key References
  • Bartek J, et. al. Oncogene 7:101-108, 1992.
  • Grand et al. 1989. Oncogene. 4(11):1291-8.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
5925
SwissProt
P06400
Chromosome Location
13q14.2
Mol. Weight of Antigen
105kDa
Alternate Names
Retinoblastoma-associated protein, p105-Rb, p110-RB1, pRb, pp110, OSRC; Osteosarcoma; p105-Rb; PP105; pp110; pRb; Prepro retinoblastoma associated protein; RB1; Retinoblastoma 1; Retinoblastoma-associated protein

Summary

Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. It shows no cross reaction with p107 or p130. It reacts with the hyper-phosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

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