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Protocadherin FAT2 (FAT Atypical Cadherin 2) Antibody [8C5]

In Stock
Catalog Number Formulation Size Price
2196-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2196-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2196-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a protein of 480kDa, which is identified as FAT2. The cadherins represent a family of Ca2+-dependent adhesion molecules that function to mediate cell-to-cell binding that is critical for the maintenance of structure and morphogenesis. Cadherins each contain a large extracellular domain at the N-terminus, which is characterized by a series of five homologous repeats, the most distal of which is thought to be responsible for binding specificity. The relatively short C-terminal intracellular domain interacts with a variety of cytoplasmic proteins, including -catenin, to regulate cadherin function. The cadherin superfamily includes cadherins, protocadherins, desmogleins and desmocollins. FAT2 (FAT tumor suppressor homolog 2) is a single-pass type I membrane protein that belongs to the protocadherin subfamily of cadherins. FAT2 contains one Laminin G-like domain, two EGF-like domains and 32 cadherin domains and is believed to function as a cell adhesion molecule, controlling cell proliferation and playing an important role in cerebellum development.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Cell membrane, Golgi apparatus, trans-Golgi network
Gene Name
Positive Control
Cervix, Esophagus, Tonsil or Cerebellum., Uterus
Immunogen
Purified recombinant human FAT2 fusion protein.
Alternate Names
Protocadherin Fat 2, Cadherin family member 8, Multiple epidermal growth factor-like domains protein 1, FAT tumor suppressor homolog 2 (HFAT2); cadherin family member 8 (CDHF8); cadherin-related family member 9 (CDHR9); multiple EGF-like domains protein 1; multiple epidermal growth factor-like domains 1 (MEGF1); multiple epidermal growth factor-like domains protein 1; protocadherin FAT2.

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
8C5
Chromosome Location
5q33.1
Mol. Weight of Antigen
480kDa

Functions

  • Involved in the regulation of cell migration (PubMed:18534823). May be involved in mediating the organization of the parallel fibers of granule cells during cerebellar development (By similarity).

Key References

  • Sano, K., et al. 1993. Protocadherins: a large family of cadherin-related molecules in central nervous system. EMBO J. 12: 2249-2256.
  • Suzuki, S.T. 2000. Recent progress in protocadherin research. Exp. Cell Res. 261: 13-18.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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