PRDM1 / BLIMP-1 Antibody [PCRP-PRDM1-2B9]

These antibodies are validated on HuProtâ„¢ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE

Additional information

Catalog Number: 639-MSM1
Host:

Reactivity:

,

Gene:

In Stock

Trial Size Available

Carrier-Free Available

In Stock
Catalog Number Formulation Size Price
639-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
639-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
639-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
  • Flow
  • IF

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml

Product Properties

Antibody Type
Host
Mouse
Species Reactivity
Clone
PCRP-PRDM1-2B9
Isotype
IgG2a
Cellular Localization
Nucleus. Cytoplasm.
Gene Name
Positive Control
HeLa or MCF-7 cells.
Immunogen
Recombinant full-length human PRDM1 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

Related Antibodies

Other Information

Functions
Key References
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
639
SwissProt
Chromosome Location
6q21
Mol. Weight of Antigen
114.8kDa
Alternate Names
PR domain zinc finger protein 1, BLIMP-1, Beta-interferon gene positive regulatory domain I-binding factor, PR domain-containing protein 1, Positive regulatory domain I-binding factor 1, B Lymphocyte Induced Maturation Protein 1 ; Beta-interferon gene positive regulatory domain I-binding factor ; BLIMP1 ; Positive Regulatory Domain I Binding Factor 1 ; PR domain containing 1 with ZNF domain isoform 2 ; PR domain zinc finger protein 1 ; PR domain-containing protein 1 ; PRDI-BF1 ; PRDI-binding factor 1

Summary

The development and differentiation of plasma cells, which are terminally differentiated B-cells, are induced by Blimp-1 (B lymphocyte-induced maturation protein, also designated PRDI-BF1). Blimp-1 is a transcriptional repressor that localizes to the nucleus and is considered a master regulator of terminal B-cell development. Alone, Blimp-1 is sufficient to trigger terminal B-cell differentiation. Blimp-1 upregulates the expression of syndecan-1 and J chain, represses IFN-b gene transcription and associates with HDAC to recruit it to DNA, thereby repressing c-Myc. Blimp-1 is expressed during the late stages of B-cell differentiation in immunoglobulin-secreting plasma cells, as well as in long-lived, bone marrow plasma cells. The expression of Blimp-1 defines a checkpoint beyond which fully activated B cells proceed to the plasma cell stage, whereas immature and partially activated cells are eliminated.

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