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PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody [13C4]

Additional information

Catalog Number: 7345-MSM2
Reactivity:

, , , , , , , , ,

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
7345-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
7345-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
7345-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
PGP9.5 / UchL1 Antibody in Western Blot (WB)

PGP9.5 / UchL1 Antibody in Western Blot (WB)

Western blot analysis of human brain tissue lysate using PGP9.5 / UchL1 Mouse Monoclonal Antibody (13C4).

UchL1 Antibody in SDS-PAGE

UchL1 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified PGP9.5 / UchL1 Mouse Monoclonal Antibody (13C4). Confirmation of Integrity and Purity of Antibody.

PGP9.5 / UchL1 (pan-Neuronal Marker) Antibody - Image 3

Immunofluorescence Analysis of T98G cells labeling Pgp9.5 with Pgp9.5 / UchL1 Mouse Monoclonal Antibody (13C4) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Nucspot (Red)

PGP9.5 / UchL1 Antibody in Western Blot (WB)

PGP9.5 / UchL1 Antibody in Western Blot (WB)

Western Blot Analysis of Human Brain, Mouse Brain, Rat Brain, Guinea pig Brain and Hamster Brain tissue lysates using PGP9.5 / UchL1 Mouse Monoclonal Antibody (13C4).

  • IF
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol
Immunofluorescence (IF) 1-3ug/ml
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunofluorescence, Western Blot
Species Reactivity
Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat, Sheep, Zebrafish
Clone
13C4
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Cytoplasm, Endoplasmic reticulum membrane
Gene Name
UCHL1
Positive Control
human brain, Cerebellum. Mouse Brain, Rat Brain, Guinea pig Brain or Hamster Brain
Immunogen
Native UchL1 (PGP9.5) protein from brain
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins (Probable). This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin (PubMed:9774100, PubMed:8639624, PubMed:12408865, PubMed:23359680). Also binds to free monoubiquitin and may prevent its degradation in lysosomes (By similarity). The homodimer may have ATP-independent ubiquitin ligase activity (PubMed:12408865).
Key References
  • Day IN et. al. Biochem Society Trans 14:350-351 (1986)
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
7345
SwissProt
P09936
Chromosome Location
4p13
Mol. Weight of Antigen
20-30kDa
Alternate Names
Ubiquitin carboxyl-terminal hydrolase isozyme L1, Neuron cytoplasmic protein 9.5, PGP 9.5, Ubiquitin thioesterase L1, Gracile Axonal Dystrophy; Neuron Cytoplasmic Protein 9.5; Park5; Parkinson Disease 5; PGP95; Protein Gene Product 9.5; Ubiquitin Carboxyl-terminal Esterase L1; Ubiquitin Carboxyl-terminal Hydrolase Isozyme L1; Ubiquitin Thioesterase L1; Ubiquitin Thiolesterase L1

Summary

This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons and neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells, and dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson’s disease.

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