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PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target) Antibody [PDL1/2746]

In Stock
Catalog Number Formulation Size Price
29126-MSM6-B1
Purified Ab conjugated to Biotin
0.5ml at 100ug/ml
$519.00
29126-MSM6-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
29126-MSM6-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
29126-MSM6-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
29126-MSM6-PE-100T
Purified Ab conjugated to PE
0.5ml at 100ug/ml
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG2b /
Cellular Localization
Cell membrane, Early endosome membrane, Endomembrane system, Recycling endosome membrane
Gene Name
Positive Control
Heart, Jurkat
Immunogen
Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)
Alternate Names
Programmed cell death 1 ligand 1, B7 homolog 1, B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
PDL1/2746
Chromosome Location
9p24.1
Mol. Weight of Antigen
37-50kDa

Functions

  • Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11015443, PubMed:28813417, PubMed:28813410). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11015443, PubMed:28813417, PubMed:28813410). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:10581077).
  • The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:28813417, PubMed:28813410). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (By similarity). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (By similarity).

Key References

  • Dong, H., et al. 1999. Nat. Med. 5: 1365-1369.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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