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Occludin (OCLN) (Tight Junctions Marker) Antibody [OCLN/2182]

In Stock
Catalog Number Formulation Size Price
100506658-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$249.00
100506658-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$539.00
100506658-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$539.00
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
Occludin (OCLN) (Tight Junctions Marker) Antibody

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2182). Inset: PBS instead of primary antibody; secondary only negative control.

Occludin (OCLN) (Tight Junctions Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2182). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Occludin (OCLN) (Tight Junctions Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2182). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

OCLN (Occludin) Antibody in SDS-PAGE

OCLN (Occludin) Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified OCLN (Occludin) Mouse Monoclonal Antibody(OCLN/2182). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial Occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
BBB VCAM-1 Signaling, Transcription Factors
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell Surface. Cytoplasm.
Gene Name
OCLN
Positive Control
HepG2 cells. Human kidney.
Immunogen
Recombinant human Occludin fragment around aa 282-415 (Exact sequence is proprietary)
Alternate Names
Occludin, BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PPP1R115; PTORCH1; Tight junction protein occludin

Database Links

Entrez Gene ID
100506658
SwissProt
Q16625

Additional Information

Clone
OCLN/2182
Chromosome Location
5q13.2
Mol. Weight of Antigen
60-82kDa

Related Antibodies

View All OCLN Antibodies

Functions

  • May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
  • (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.

Key References

  • Furuse, M., et al. 1993. Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell Biol. 123: 1777-1788.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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