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Occludin (OCLN) (Tight Junctions Marker) Antibody [OCLN/2182]

Additional information

Catalog Number: 100506658-MSM2
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
100506658-MSM2-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
100506658-MSM2-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
100506658-MSM2-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Occludin (OCLN) (Tight Junctions Marker) Antibody

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2182). Inset: PBS instead of primary antibody; secondary only negative control.

Occludin (OCLN) (Tight Junctions Marker) Antibody - Image 2

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2182). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Occludin (OCLN) (Tight Junctions Marker) Antibody - Image 3

Formalin-fixed, paraffin-embedded human kidney stained with Occludin Mouse Monoclonal Antibody (OCLN/2182). HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

OCLN (Occludin) Antibody in SDS-PAGE

OCLN (Occludin) Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified OCLN (Occludin) Mouse Monoclonal Antibody(OCLN/2182). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
OCLN/2182
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell Surface. Cytoplasm.
Gene Name
OCLN
Positive Control
HepG2 cells. Human kidney.
Immunogen
Recombinant human Occludin fragment around aa 282-415 (Exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability barrier. It is able to induce adhesion when expressed in cells lacking tight junctions.
  • (Microbial infection) Acts as a coreceptor for hepatitis C virus (HCV) in hepatocytes.
Key References
  • Furuse, M., et al. 1993. Occludin: a novel integral membrane protein localizing at tight junctions. J. Cell Biol. 123: 1777-1788.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
100506658
SwissProt
Q16625
Chromosome Location
5q13.2
Mol. Weight of Antigen
60-82kDa
Alternate Names
Occludin, BLCPMG; Occludin; OCLN; Phosphatase 1 regulatory subunit 115; PPP1R115; PTORCH1; Tight junction protein occludin

Summary

Occludin is a tetraspan integral membrane protein in epithelial and endothelial tight junction (TJ) structures that can contain two extracellular loops. The protein exists in a variety of phosphorylated forms. Phosphorylation is involved in regulating both the localization and the function of Occludin. Expression of Occludin is upregulated by polyunsaturated fatty acids, increasing trans-endothelial cell resistance and reducing cellular permeability to large molecules. The level of Occludin varies greatly depending on tissue; in brain tissue, Occludin is highly expressed at cell-cell contact sites. Non-neural tissues show lower expression and discontinuous distribution. Up-regulation of epithelial Occludin may play a role in enhancing paracellular permeability and be related to the damage to the tight junction.

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