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Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/1902).
Formalin-fixed, paraffin-embedded human colon carcinoma stained with Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/1902).
Western Blot Analysis of A431 cell lysate using Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/1902).
Western Blot Analysis of K562 cell lysate using Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/1902).
Immunofluorescence staining of HeLa cells using Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/1902) followed by goat anti-mouse IgG-CF488 (green). Phalloidin counterstain.
Analysis of Protein Array containing more than 19,000 full-length human proteins using Nucleophosmin-Monospecific Mouse Monoclonal Antibody (NPM1/1902)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Western blot analysis of human heart, kidney, lung and pancreas tissue lysates using NPM1 Mouse Monoclonal Antibody (NPM1/1902).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
| Western Blot (WB) | 2-4ug/ml | Western Blot Protocol |
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Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia.The antibody may be a useful aid for classification of acute myeloid leukemia.
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