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  • HuProt™ Validated

Nucleophosmin (Acute Myeloid Leukemia Marker) Antibody [NPM1/3285]

These antibodies are validated on HuProt™ Human Protein Microarrays containing over 21,000 full-length human proteins, including the specific target of interest. LEARN MORE

Additional information

Catalog Number: 4869-MSM5-IHC7
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

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In Stock
Catalog Number Formulation Size Price
4869-MSM5-IHC7
7.0ml of Prediluted Antibody for IHC
7.0ml
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
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Nucleophosmin Antibody in Immunohistochemistry (IHC (P))

Nucleophosmin Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human skin stained with Nucleophosmin Mouse Monoclonal Antibody (NPM1/3285). Inset: PBS instead of primary antibody; secondary only negative control.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
NPM1/3285
Isotype
IgG2c
Light Chain
Kappa
Cellular Localization
Centrosome, Cytoplasm, Cytoskeleton, Microtubule organizing center, Nucleolus, Nucleoplasm, Nucleus
Gene Name
NPM1
Positive Control
Human skin or colon.
Immunogen
Recombinant human NPM1 protein fragment (aa185-287) (exact sequence is proprietary)
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
7.0ml of Ready-to-Use for IHC staining of formalin-fixed paraffin-embedded tissues.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade (PubMed:22528486). In complex with MYC enhances the transcription of MYC target genes (PubMed:25956029).
Key References
  • Nozawa, Y., et al. 1996. Expression of nucleophosmin/B23 in normal and neoplastic colorectal mucosa. J. Pathol. 178: 48-52.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4869
SwissProt
P06748
Chromosome Location
5q35.1
Mol. Weight of Antigen
33kDa
Alternate Names
Nucleophosmin, Nucleolar phosphoprotein B23, Nucleolar protein NO38, Numatrin, NO38; NPM; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin (nucleolar phosphoprotein B23 numatrin); Nucleophosmin; Nucleophosmin/nucleoplasmin family member 1; Numatrin

Summary

Recognizes a 33kDa glycoprotein, identified as Nucleophosmin (NPM). It is predominantly localized in the nucleus of cells in most tissues. NPM is involved in ribosomal assembly and rRNA transport. It is an abundant protein that is highly phosphorylated by Cdc2 kinase during mitosis. This phosphoprotein moves between the nucleus and the cytoplasm. It is thought to be involved in several processes including regulation of the ARF/p53 pathway. A number of genes are fusion partners, in particular the anaplastic lymphoma kinase gene on chromosome 2. Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location. Mutations in this gene are associated with acute myeloid leukemia. The antibody may be a useful aid for classification of acute myeloid leukemia.

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