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Natriuretic Peptide B / NPPB Antibody [NPPB/4493]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
4879-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
4879-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
4879-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human heart stained with Natriuretic Peptide BMouse Monoclonal Antibody (NPPB/4493).

Formalin-fixed, paraffin-embedded human heart stained with Natriuretic Peptide BMouse Monoclonal Antibody (NPPB/4493).

Inset: PBS instead of primary antibody; secondary only negative control.

Inset: PBS instead of primary antibody; secondary only negative control.

Formalin-fixed, paraffin-embedded human uterusstained with Natriuretic Peptide B Mouse Monoclonal Antibody (NPPB/4493).

Formalin-fixed, paraffin-embedded human uterusstained with Natriuretic Peptide B Mouse Monoclonal Antibody (NPPB/4493).

HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

HIER: Tris/EDTA, pH9.0, 45min. 2°C: HRP-polymer, 30min. DAB, 5min.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Natriuretic peptides comprise a family of three structurally related molecules: atrial natriuretic peptide (ANP), brain natriuretic peptide (BNP) and C-type natriuretic peptide (CNP). ANP and BNP act mainly as cardiac hormones, produced primarily by the atrium and ventricle, respectively, while the gene encoding C-type natriuretic peptide is expressed mainly in the brain. These peptides possess potent natriuretic, diuretic and vasodilating activities and are implicated in body fluid homeostasis and blood pressure control. ANP, BNP and CNP are highly homologous within the 17-residue ring structure formed by an intramolecular disulfide linkage. The genes which encode for ANP and BNP map to human chromosome 1p36.22. The gene which encodes for CNP maps to human chromosome 2q37.1.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Cardiovascular
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Secreted
Gene Name
NPPB
Positive Control
Human heart, liver or uterus.
Immunogen
Recombinant fragment of human NPPB (exact sequence is proprietary)
Alternate Names
Natriuretic peptides B, Brain natriuretic factor prohormone, Gamma-brain natriuretic peptide, Iso-ANP, Brain natriuretic peptide 32; Brain type natriuretic peptide; Gamma brain natriuretic peptide; Natriuretic peptide B; Natriuretic peptide precursor B; natriuretic peptide, brain type; Natriuretic peptides B; natriuretic protein; NPPB

Database Links

Entrez Gene ID
4879
SwissProt
P16860

Additional Information

Clone
NPPB/4493
Chromosome Location
1p36.22
Mol. Weight of Antigen
25-36kDa (glycosylated BNP precursor); 12kDa (deglycosylated mature BNP)

Related Antibodies

View All NPPB Antibodies

Functions

  • Cardiac hormone that plays a key role in mediating cardio-renal homeostasis (PubMed:9458824, PubMed:1672777, PubMed:1914098, PubMed:17372040). May also function as a paracrine antifibrotic factor in the heart (By similarity). Acts by specifically binding and stimulating NPR1 to produce cGMP, which in turn activates effector proteins that drive various biological responses (PubMed:9458824, PubMed:1672777, PubMed:17372040, PubMed:21098034, PubMed:17349887, PubMed:25339504). Involved in regulating the extracellular fluid volume and maintaining the fluid-electrolyte balance through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion (PubMed:9458824, PubMed:1914098). Binds the clearance receptor NPR3 (PubMed:16870210).
  • May affect cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP although its potency is very low compared to brain natriuretic peptide 32 (PubMed:17372040).
  • May have a role in cardio-renal homeostasis (PubMed:17372040). Able to promote the production of cGMP (PubMed:17372040).

Key References

  • Mair, J., et al. 2001. The impact of cardiac natriuretic peptide determinationon the diagnosis and management of heart failure. Clin. Chem. Lab. Med.39: 571-588.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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