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Myeloperoxidase / MPO Antibody [MPO/7118]

These antibodies perform exceptionally well in the intended applications and have undergone stringent testing and additional validation to ensure monospecificity.

The MAbMan recommends these antibodies for repeatable and reliable results.

Additional information

Catalog Number: 4353-MSM8
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4353-MSM8-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4353-MSM8-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4353-MSM8-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Myeloperoxidase / MPO Antibody

Formalin-fixed, paraffin-embedded human bone marrow stained with Myeloperoxidase Mouse Monoclonal Antibody (MPO/7118).

Myeloperoxidase / MPO Antibody - Image 2

Formalin-fixed, paraffin-embedded human bone marrow stained with Myeloperoxidase Mouse Monoclonal Antibody (MPO/7118). HIER: Tris/EDTA, pH9.0, 45min. 2 °: HRP-polymer, 30min. DAB, 5min.

Myeloperoxidase / MPO Antibody in Western Blot (WB)

Myeloperoxidase / MPO Antibody in Western Blot (WB)

Western blot analysis of human spleen tissue lysate using MPO Mouse Monoclonal Antibody (MPO/7118).

Myeloperoxidase / MPO Antibody in SDS-PAGE

Myeloperoxidase / MPO Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified MPO Mouse Monoclonal Antibody (MPO/7118) of Purity and Integrity of Antibody.

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
MPO/7118
Isotype
IgG2a
Light Chain
Kappa
Cellular Localization
Lysosome
Gene Name
MPO
Positive Control
Human B cell lymphoma or liver tissue. Spleen.
Immunogen
Recombinant fragment of human MPO protein (exact sequence is proprietary)
Expression System
HEK293
Purification
Protein G
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity (PubMed:9922160). Mediates the proteolytic cleavage of alpha-1-microglobulin to form t-alpha-1-microglobulin, which potently inhibits oxidation of low-density lipoprotein particles and limits vascular damage (PubMed:25698971).
Key References
  • Toth B, Wehrmann M,et al. J Clin Pathol 1999 Sep;52(9):688-92.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4353
SwissProt
P05164
Chromosome Location
17q22
Mol. Weight of Antigen
heavy-light promoter: 72kDa; dimer: 140kDa
Alternate Names
Myeloperoxidase, 84 kDa / 89 kDa myeloperoxidase; EC 1.11.1.7; EC1.11.2.2; fj80f04; MPO; mpx; myeloid-specific peroxidase; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain

Summary

Myeloperoxidase (MPO) also called the peroxidase (POD), is an important marker of bone marrow cells. It is one of the members of the family of heme peroxidase super existing in myeloid cells (mainly neutrophils and monocytes of aniline blue particles). With the deepening of the research on MPO, MPO gene polymorphism has been found to lead to individual for some disease susceptibility differences, with a variety of human development is closely related to the occurrence of diseases. The antibody reacts with neutrophil granulocytes and monocytes in blood and with precursors of granulocytes in the bone marrow. The antibody is useful as an aid for classification of neoplastic tissue, i.e. myeloblasts and immature myeloid cells of acute myelogenous leukemia, progranulocytic leukemia, monomyelocytic leukemia, erythroleukemia and myeloblastoma.

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