Myeloid Cell Marker (Macrophage / Granulocyte Marker) [SPM250]

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Summary

Recognizes an unidentified antigen in the cytoplasm of mature granulocytes. It shows no reactivity with any other cell type in human tissues. Markers of myeloid cells are useful in the identification of different levels of cellular differentiation. MAb BM-2 reacts with early precursor and mature forms of human myeloid cells. This MAb is useful for the detection of myeloid leukemias and granulocytic sarcomas. It can be used as a marker of granulocytes in normal tissues or inflammatory processes.

Product Properties & Targets

Host
Species Reactivity
Isotype
Cellular Localization
N/A
Gene Name
N/A
Mol. Weight of Antigen
Unknown
Immunogen
Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes.

Database Links

Entrez Gene ID
Unigene
SwissProt
N/A

Additional Information

Clone
SPM250

Known Applications & Suggested Dilutions

  • ELISA (For coating, order Ab without BSA)
  • Flow Cytometry (1-2ug/million cells)
  • Immunofluorescence (1-2ug/ml)
  • Immunohistochemistry (Frozen & Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (No special pretreatment is required for staining of formalin/paraffin tissues.)Optimal dilution for a specific application should be determined.

Key References

  • de Swart RL et al. Predominant infection of CD150+ lymphocytes and dendritic cells during measles virus infection of macaques. PLoS Pathog 3:e178 (2007).

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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