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MUC5AC (Mucin 5AC / Gastric Mucin) Antibody [SPM297]

Additional information

Catalog Number: 4586-MSM1X
Reactivity:

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Gene:

Host:

In Stock

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Best Seller
Catalog Number Formulation Size Price
4586-MSM1X-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4586-MSM1X-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4586-MSM1X-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
MUC5AC (Mucin 5AC / Gastric Mucin) Antibody

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (SPM297).

MUC5AC Antibody in SDS-PAGE

MUC5AC Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified Mucin-5AC Mouse Monoclonal Antibody (SPM297). Confirmation of Purity and Integrity of Antibody.

  • Flow
  • IF
  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Cat, Chicken, Hedgehog, Human, Monkey, Mouse, Pig, Rabbit, Rat
Clone
SPM297
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Secreted
Gene Name
MUC5AC
Positive Control
Human colon or stomach (IHC).
Immunogen
M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Gel-forming glycoprotein of gastric and respiratory tract epithelia that protects the mucosa from infection and chemical damage by binding to inhaled microorganisms and particles that are subsequently removed by the mucociliary system (PubMed:14535999, PubMed:14718370). Interacts with H.pylori in the gastric epithelium, Barrett's esophagus as well as in gastric metaplasia of the duodenum (GMD) (PubMed:14535999).
Key References
  • Bara J et. al. International Journal of Cancer, 1991, 47(2):304-10.
  • Bara J et. al. Journal of Immunological Methods, 1992, 149(1):105-13.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4586
SwissProt
P98088
Chromosome Location
11p15.5
Mol. Weight of Antigen
>1|000kDa
Alternate Names
Mucin-5AC, Gastric mucin, Major airway glycoprotein, Mucin-5 subtype AC, tracheobronchial, Tracheobronchial mucin, Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)

Summary

This MAb recognizes the peptide core of gastric mucin M1 (1,000kDa) (recently identified as Mucin 5AC). Its epitope is destroyed by beta-mercaptoethanol and proteases but not by periodate treatment. Antibody to gastric mucin M1 reacts with the gastric epithelium of normal human gastrointestinal tract as well as with the precancerous and cancerous colon but not with normal adult colon. It also reacts with fetal colonic mucosa. Resurgence of gastric mucin reactivity during colonic carcinogenesis is due to re-expression of the peptide core of gastric (or fetal colonic) mucins.

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