Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Recombinant Migfilin-1 / FBLIM1 Antibody [FBLIM1/8189R]

In Stock
Catalog Number Formulation Size Price
54751-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
54751-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
54751-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Kindlin-2 (also known as Mig-2, Migfilin) is an important component of cell-extracellular matrix adhesion. Kindlin-2 binds integrins and is involved in ECM adhesion, actin assembly and cell shape modulation. This monoclonal antibody recognizes kindlin-2 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

Product Properties & Targets

Host
Rabbit
Applications
Species Reactivity
Isotype / Light Chain
IgG / Kappa
Cellular Localization
Cytoplasm.
Gene Name
Positive Control
Human heart or kidney tissue.
Immunogen
Recombinant full-length human FBLIM1 protein
Alternate Names
Filamin-binding LIM protein 1, Migfilin, Mitogen-inducible 2-interacting protein, CSX associated LIM (Cal); Filamin binding LIM protein 1 (FBLIM1); Filamin-binding LIM protein 1 (FBLP1); Gt10; MIG2-interacting protein; Migfilin; Mitogen inducible 2 interacting protein; Mitogen-inducible 2-interacting protein

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
FBLIM1/8189R
Chromosome Location
14q22.1
Mol. Weight of Antigen
41kDa

Functions

  • Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion.

Key References

  • Yizeng et al. Cell (2003) 113(1): 37-47.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Recombinant Migfilin-1 / FBLIM1 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK