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Recombinant Migfilin-1 / FBLIM1 Antibody [FBLIM1/8189R]

Additional information

Catalog Number: 54751-RBM3
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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Recombinant Migfilin-1 / FBLIM1 Antibody

Formalin-fixed, paraffin-embedded human stomach stained with Migfilin-1 Recombinant Rabbit Monoclonal Antibody (FBLIM1/8189R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

  • IHC
In Stock
Catalog Number Formulation Availability Size Price
54751-RBM3-P0
Purified Ab with BSA and Azide at 200ug/ml
Trial Size
In Stock
20ug
$259.00
Trial Size Available In-Stock
54751-RBM3-P1
Purified Ab with BSA and Azide at 200ug/ml
In Stock
100ug
$559.00
In-Stock
54751-RBM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Carrier-Free
In Stock
100ug
$559.00
Carrier-Free Available In-Stock
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
FBLIM1/8189R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm.
Gene Name
FBLIM1
Positive Control
Human heart or kidney tissue.
Immunogen
Recombinant full-length human FBLIM1 protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Additional information

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Reactivity:

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Additional information

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Reactivity:

, , , ,

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Host:

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Additional information

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Host:

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Additional information

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Gene:

Host:

Reactivity:

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Other Information

Functions
  • Serves as an anchoring site for cell-ECM adhesion proteins and filamin-containing actin filaments. Is implicated in cell shape modulation (spreading) and motility. May participate in the regulation of filamin-mediated cross-linking and stabilization of actin filaments. May also regulate the assembly of filamin-containing signaling complexes that control actin assembly. Promotes dissociation of FLNA from ITGB3 and ITGB7. Promotes activation of integrins and regulates integrin-mediated cell-cell adhesion.
Key References
  • Yizeng et al. Cell (2003) 113(1): 37-47.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
54751
SwissProt
Q8WUP2
Chromosome Location
14q22.1
Mol. Weight of Antigen
41kDa
Alternate Names
Filamin-binding LIM protein 1, Migfilin, Mitogen-inducible 2-interacting protein, CSX associated LIM (Cal); Filamin binding LIM protein 1 (FBLIM1); Filamin-binding LIM protein 1 (FBLP1); Gt10; MIG2-interacting protein; Migfilin; Mitogen inducible 2 interacting protein; Mitogen-inducible 2-interacting protein

Summary

Kindlin-2 (also known as Mig-2, Migfilin) is an important component of cell-extracellular matrix adhesion. Kindlin-2 binds integrins and is involved in ECM adhesion, actin assembly and cell shape modulation. This monoclonal antibody recognizes kindlin-2 in multiple assays including Western blotting, immunoprecipitation, immunohistochemical and immunofluorescent staining.

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