Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

MECA-79 Antibody [MECA-79]

In Stock
Catalog Number Formulation Size Price
RTM1-1599-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
RTM1-1599-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
RTM1-1599-P1BX
Purified Ab WITHOUT BSA at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml

Summary

MECA-79 recognizes a carbohydrate epitope shared with a group of sulfation decorated sialomucins, including sulfated ligands for CD62L (CD34, GlyCAM-1, Sgp200, and a subset of MAdCAM-1). This set antigens has been referred to as peripheral node addressin (PNAd) with the molecular mass 50-250 kD. It has been identified that GlcNAc-6-SO4 sulfation contributes to MECA-79 binding and the core 1 beta1,3-N-acetylglucosaminyltransferase is required for the generation of the MECA-79 epitope. MECA-79 is expressed on high endothelial venules (HEV) of lymphoid tissues, chronic inflamed tissues and rheumatoid synovia. The interaction of PNAd with CD62L receptor is involved in tethering and rolling of lymphocytes along HEV in lymphoid tissues. MECA-79 antibody reacts with Mouse, human and many other species PNAd and blocks L-selectin-dependent lymphocyte adhesion in vitro and in vivo.

Product Properties & Targets

Antibody Type
Host
Rat
Species Reactivity
Isotype / Light Chain
IgM / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
Human or mouse tonsil.
Immunogen
Mouse lymph node stromal cells
Alternate Names
N/A, N/A, MECA-79; Peripheral Node Addressin (PNAd)

Database Links

Entrez Gene ID
SwissProt
N/A

Additional Information

Clone
MECA-79
Chromosome Location
Not Known
Mol. Weight of Antigen
90-110kDa

Functions

  • N/A

Key References

  • Bistrup, A., et al. 2004. Am. J. Pathol. 164: 1635-164

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “MECA-79 Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK