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MCM3 (Proliferation Marker) Antibody [MSVA-503M]

In Stock
Catalog Number Formulation Size Price
4172-MSM7-P0
Purified Ab with BSA and Azide
20ug
$249.00
4172-MSM7-P1
Purified Ab with BSA and Azide
100ug
$544.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
MCM3 Antibody in Numerous Tissues (IHC)

MCM3 Antibody in Numerous Tissues (IHC)

DNA replication licensing factor MCM3 Mouse Monoclonal Antibody (MSVA-503M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for DNA replication and cell proliferation.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Research Areas
Nuclear Marker
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Nucleus
Gene Name
MCM3
Positive Control
Colon: A strong nuclear MCM3 immunostaining should be seen in virtually all crypt base cells.
Immunogen
Recombinant fragment (aa 650-750) of human MCM3 protein (exact sequence is proprietary)
Alternate Names
DNA replication licensing factor MCM3, DNA polymerase alpha holoenzyme-associated protein P1, P1-MCM3, RLF subunit beta, p102, Cervical cancer proto-oncogene 5; DNA polymerase alpha holoenzyme-associated protein P1; DNA replication factor MCM3; DNA replication licensing factor mcm3; HCC5; hRlf beta subunit; Human cervical cancer proto oncogene 5; MGC1157; Minichromosome maintenance complex component 3; Minichromosome maintenance deficient 3; P102 protein; Replication licensing factor beta subunit; RLF beta subunit; RLF subunit beta

Database Links

Entrez Gene ID
4172
SwissProt
P25205

Additional Information

Clone
MSVA-503M
Chromosome Location
6p12.2
Mol. Weight of Antigen
115kDa

Related Antibodies

View All MCM3 Antibodies

Functions

  • Acts as component of the MCM2-7 complex (MCM complex) which is the replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. Core component of CDC45-MCM-GINS (CMG) helicase, the molecular machine that unwinds template DNA during replication, and around which the replisome is built (PubMed:32453425, PubMed:34694004, PubMed:34700328, PubMed:35585232). The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (PubMed:32453425). Required for the entry in S phase and for cell division (Probable).

Key References

  • Koonin, E.V. 1993. Nucleic Acids Res. 21: 2541-2547.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in CHO cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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