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Recombinant MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody [MLANA/8365R]

Additional information

Catalog Number: 2315-RBM23
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2315-RBM23-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2315-RBM23-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2315-RBM23-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Recombinant MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody

Formalin-fixed, paraffin-embedded human melanoma stained with MART-1 Recombinant Rabbit Monoclonal Antibody (MLANA/8365R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.

Recombinant MART-1 / Melan-A / MLANA (Melanoma Marker) Antibody - Image 2

SDS-PAGE Analysis of Purified MART-1 Recombinant Rabbit Monoclonal Antibody (MLANA/8365R). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Recombinant Rabbit Monoclonals
Host
Rabbit
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
MLANA/8365R
Isotype
IgG
Light Chain
Kappa
Cellular Localization
Cytoplasm.
Gene Name
MLANA
Positive Control
Human skin or melanoma.
Immunogen
Recombinant fragment corresponding to the N-terminus of human Melan A protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Functions
  • Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes.
Key References
  • Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25.
  • Marincola FM, et. al. Journal of Immunotherapy with Emphasis on Tumor Immunology, 1996, 19(3):192-205.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2315
SwissProt
Q16655
Chromosome Location
9p24.1
Mol. Weight of Antigen
20-22kDa (doublet)
Alternate Names
Melanoma antigen recognized by T-cells 1, Antigen LB39-AA, Antigen SK29-AA, Protein Melan-A, Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1 (MART-1), MLAN-A, MLANA

Summary

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

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