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Lewis Y (Tumor Marker) Antibody [A70-A/A9]

In Stock
Catalog Number Formulation Size Price
MSM2-1412-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM2-1412-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM2-1412-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Lewis Y Mouse Monoclonal Antibody (A70-A/A9).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Lewis Y Mouse Monoclonal Antibody (A70-A/A9).

SDS-PAGE Analysis of Purified Lewis Y Mouse Monoclonal Antibody (A70-A/A9). Confirmation of Purity and Integrity of Antibody.

SDS-PAGE Analysis of Purified Lewis Y Mouse Monoclonal Antibody (A70-A/A9). Confirmation of Purity and Integrity of Antibody.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This antibody recognizes a carbohydrate epitope present on tumor-associated Lewis Y antigen (Fucalpha1-2Galbeta1-4/3[Fucalpha1-3/4]GlcNAcbeta-). Lewis Y is expressed in large bowel tumors and colorectal carcinomas. It may be useful in the classification of human renal and bladder tumors. The Lewis Y antigen has been evaluated as a clinical marker for the diagnosis and prognosis of cholangiocarcinoma, hepatocellular carcinoma and breast cancer.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
Bladder or Hepatocellular Carcinoma., Colon, Lung
Immunogen
Live Ls174T cells (human colon carcinoma cell line)
Alternate Names
N/A, N/A, Lewis Y antigen

Database Links

Entrez Gene ID
Not Applicable
SwissProt
N/A

Additional Information

Clone
A70-A/A9
Chromosome Location
Not Applicable
Mol. Weight of Antigen
Multiple

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Christensen PA, Danielczyk A, Ravn P, Stahn R, Karsten U, Goletz S. A monoclonal antibody to Lewis Y/Lewis b revealing mimicry of the histone H1 to carbohydrate structures. Scand J Immunol. 2007 Apr;65(4):362-7.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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