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Lewis A (Blood Group Antigen) Antibody [SPM522]

In Stock
Catalog Number Formulation Size Price
MSM1XX-311-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM1XX-311-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM1XX-311-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Lewis A Monoclonal Antibody (SPM522).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Lewis A Monoclonal Antibody (SPM522).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Recognizes a carbohydrate determinant of Gal 1-3(Fuc 1-4) GlcNAc which is blood group antigen Lewis A. It is present primarily on epithelial cells such as colon and kidneys. In the tumors and dedifferentiated tissues, decrease of Lewis A antigen was observed. Lewis A (type 1 chain) is expressed in colonic epithelial cells and may be useful for detection of gastrointestinal tumors, pancreatic cancer, and colorectal tumors. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry
Species Reactivity
Human, Mouse
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
N/A
Gene Name
N/A
Positive Control
Colon tissue.
Immunogen
Mucins isolated from ovarian cyst fluid
Alternate Names
N/A, N/A, Lewis A antigen

Database Links

Entrez Gene ID
Not Applicable
SwissProt
N/A

Additional Information

Clone
SPM522
Chromosome Location
Not Applicable
Mol. Weight of Antigen
Multiple

Related Antibodies

View All N/A Antibodies

Functions

  • N/A

Key References

  • Gastroenterology, 102 (2), 424-430 (1992).
  • Blood transfusion and immunohaematology, Ph. Rouger, D Anstee and Ch. Salmon (Eds). Arnette, France 30 (5): 353-720 (1987).
  • Cancer Epidemiology, Biomarkers Prevention 1, 199-205 (1992).

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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