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Interleukin-3 (IL-3) Antibody [IL3/4004]

In Stock
HuProt Validated
Catalog Number Formulation Size Price
3562-MSM4-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
3562-MSM4-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
3562-MSM4-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 not only supports growth of both pluripotent stem cells and the more differentiated committed progenitors, but it also stimulates the functional activity of some fully differentiated cells. IL-3 has also been shown to protect mast cells from undergoing apoptosis. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific 伪 subunit and a signal transducing 尾 subunit common to the IL-3/IL-5/GM-CSF receptors. The carboxy terminus of the 尾 subunit has been shown to be necessary for activation of the MAP kinase signaling pathway. Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b.

Product Properties & Targets

Antibody Type
Host
Mouse
Applications
Species Reactivity
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Secreted
Gene Name
IL3
Positive Control
Human thymus or tonsil tissue.
Immunogen
Recombinant fragment of human IL3 protein (exact sequence is proprietary)
Alternate Names
Interleukin-3, Hematopoietic growth factor, Mast cell growth factor, Multipotential colony-stimulating factor, P-cell-stimulating factor, Colony stimulating factor multiple; Hematopoietic growth factor; Interleukin 3 (colony stimulating factor, multiple); Mast cell growth factor; MCGF; Multi CSF; Multilineage colony stimulating factor; P cell stimulating factor

Database Links

Entrez Gene ID
SwissProt

Additional Information

Clone
IL3/4004
Chromosome Location
5q31.1
Mol. Weight of Antigen
17kDa

Functions

  • Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages.
  • This CSF induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes.

Key References

  • Strauchen JA, Breakstone BA. IL-2 receptor expression in human lymphoid lesions. Immunohistochemical study of 166 cases. Am J Pathol. 1987;126(3):506 512.

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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