Interferon gamma (IFNG) Antibody [IFNG/466]

Name: Interferon gamma (IFNG) Antibody
SKU: 3458-MSM4-B1
Price: 519.00 USD
Availability: InStock

In Stock

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Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Interferon gamma Mouse Monoclonal Antibody (IFNG/466).

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Interferon gamma Mouse Monoclonal Antibody (IFNG/466).

SDS-PAGE Analysis Purified Interferon gamma Mouse Monoclonal Antibody (IFNG/466). Confirmation of Purity and Integrity of Antibody

Applications & Dilutions

Flow Cytometry (Flow)

1-2ug/million cells

Flow Cytometry Protocol

Immunofluorescence (IF)

1-3ug/ml

Immunohistochemistry (IHC)

1-2ug/ml

IHC Protocol

30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

Interferon (IFN)- translated precursor is 166 amino acids, including the 23 amino acid secretory sequence. Multiple forms exist due to variable glycosylation and under non-denaturing conditions due to dimers and tetramers.

Product Properties & Targets

Antibody Type

Mouse Monoclonals

Host

Mouse

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

Species Reactivity

Human

Research Areas

Cardiovascular, Immunology, Colon Cancer, Complement System, Cytokine Signaling, Hematopoietic Stem Cells, Neuroinflammation, PD-1 blockade immunotherapy, Transcription Factors

Isotype / Light Chain

IgG2b /

Cellular Localization

Secreted

Gene Name

IFNG

Positive Control

PMA stimulated human peripheral blood mononuclear cells. Renal or testicular carcinoma.

Immunogen

Purified recombinant human full-length IFNG protein

Alternate Names

Interferon gamma, Immune interferon, AMCF1; Beta thromboglobulin like protein; CXC chemokine ligand 8 (CXCL8); Emoctakin; Granulocyte chemotactic protein 1 (GCP1); IFG; IFI; IFN Immune; IFN-gamma (IFNG); Interleukin 8; Interferon gamma; LECT; LUCT; Lymphocyte derived neutrophil activating factor; LYNAP; Macrophage Activating Factor (MAF); Monocyte derived neutrophil activating protein (MONAP); Monocyte derived neutrophil chemotactic factor (MDNCF); Neutrophil activating factor (NAF); Neutrophil activating peptide 1 (NAP1); Neutrophil activating protein 1 (NAP1); SCYB8; T cell chemotactic factor; T Cell Interferon

Database Links

Entrez Gene ID

3458

SwissProt

P01579

Additional Information

Clone

IFNG/466

Chromosome Location

12q14

Mol. Weight of Antigen

20-25kDa

Functions

Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation (PubMed:16914093, PubMed:8666937). Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation (PubMed:8349687). Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription (PubMed:16914093). Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits (PubMed:8666937). In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading (PubMed:8163024). Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference (PubMed:11112687). Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL (PubMed:7729559). Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity).

Key References

Vilcek J. Cytokine Growth Factor Rev 1997,8(4):239
Farrar MA and Schreiber RD. Annu Rev Immunol 1993, 11:571-611
Vilcek J., et al. Methods Enzymol 1986,119:48-54

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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Interferon gamma (IFNG) Antibody [IFNG/466]

Applications & Dilutions

Summary

Product Properties & Targets

Database Links

Additional Information

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