Printable Datasheet

Recombinant IgD (Immunoglobulin Delta Heavy Chain) (B-Cell Marker) [IGHD/8367R]

Clear

Summary

Immunoglobulins are four-chain, Y-shaped, monomeric structures comprised of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. The chains form two domains, the Fab (antigen binding) fragment and the Fc (constant) fragment. Immunoglobulin D (IgD) exists as a monomer with Î � heavy chains and either Î � or Î � light chains. It plays a biological role as a transmembrane receptor molecule, co-expressed with IgM on the surface of mature/naive B cells. In particular, it is found on spleen B cell surfaces. Compared to IgM, IgD exists in much lower numbers and is not expressed on immature B cells. IgD surface expression on B cells is regulated in part by IL-27. In mice, the inhibition of this immunoglobulin isotype does not cause a significant change to the immune system.

Product Properties & Targets

Host
Rabbit
Species Reactivity
Human
Isotype
IgG, kappa
Cellular Localization
Cytoplasm.
Gene Name
IGHD
Positive Control
Human tonsil lysate or NAMALWA cell lysate. Human tonsil and lymphoid tissues
Immunogen
Recombinant fragment (around aa1-200) of human IGHD protein (exact sequence is proprietary)
Alternate Names
Immunoglobulin heavy constant delta, Ig delta chain C region, Ig delta chain C region NIG-65, Ig delta chain C region WAH, Ig delta chain C region; IGHD; Immunoglobulin heavy constant delta

Database Links

Entrez Gene ID
3495
Unigene
510635
SwissProt
P01880

Additional Information

Clone
IGHD/8367R
Human Gene Symbol
IGHD
Chromosome Location
14q13
Mol. Weight of Antigen
44-80kDa (heavy chain); 21-25kDa (kappa light chain)

Functions

  • Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). IgD is the major antigen receptor isotype on the surface of most peripheral B-cells, where it is coexpressed with IgM. The membrane-bound IgD (mIgD) induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. Soluble IgD (sIgD) concentration in serum below those of IgG, IgA, and IgM but much higher than that of IgE. IgM and IgD molecules present on B cells have identical V regions and antigen-binding sites. After the antigen binds to the B-cell receptor, the secreted form sIgD is shut off. IgD is a potent inducer of TNF, IL1B, and IL1RN. IgD also induces release of IL6, IL10, and LIF from peripheral blood mononuclear cells. Monocytes seem to be the main producers of cytokines in vitro in the presence of IgD (PubMed:8774350, PubMed:10702483, PubMed:11282392).

Known Applications & Suggested Dilutions

  • Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95&degC followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Key References

  • Takayasu, T., et al. 1980. Biophys. Res. Commun. 97: 635-641.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “IgD (Immunoglobulin Delta Heavy Chain) (B-Cell Marker)”

Your email address will not be published. Required fields are marked *

Related Antibodies

View All IGHD Antibodies

Recombinant mouse monoclonal antibodies and recombinant rabbit monoclonals to support your research

Shop Primary Antibodies

Company Info
Antibodies
View All
Hosts
View All
Isotype
View All
Resources
Newsletter Signup NeoBiotechnologies
Updates & Sales

$0.00
510-376-5603