Trial Size Available
Carrier-Free Available
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Nucleolar Mouse Monoclonal Antibody (NM95).
Formalin-fixed, paraffin-embedded human Tonsil stained with Nucleolar Mouse Monoclonal Antibody (NM95).
Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CF488 Conjugate of Nucleolar Mouse Monoclonal Antibody (NM95).
Flow Cytometry of Human Nucleolar Ag on 293T cells. Black: cells alone; Grey: Isotype Control; Green: AF488-labeled human Nucleolar MAb (NM95).
Flow Cytometric Analysis of PFA-fixed K562 cells using Nucleolar Mouse Monoclonal Antibody (NM95) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)
Immunofluorescence Analysis of PFA-fixed K562 cells labeling Nucleolin with Nucleolar Mouse Monoclonal Antibody (NM95) followed by Goat anti-Mouse IgG-CF488 (Green). Membrane is stained with Phalloidin-CF640.
SDS-PAGE Analysis of Purified NM95 Mouse Monoclonal Antibody (NM95). Confirmation of Purity and Integrity of Antibody.
Western blot analysis of K-562 cell lysate using NM95 Mouse Monoclonal Antibody (NM95).
| Document Name | |
|---|---|
| Datasheet | Download Here |
| Material Safety Data Sheet | Download Here |
| Applications | Tested Dilutions | Protocol | Note |
|---|---|---|---|
| Flow Cytometry (Flow) | 1-2ug/million cells | Flow Cytometry Protocol | |
| Immunofluorescence (IF) | 1-3ug/ml | ||
| Immunohistochemistry (IHC) | 1-2ug/ml | IHC Protocol | 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes |
3
0
4
1
3
6
This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Should a NeoBiotechnologies antibody not meet the experimental expectations outlined on our website or datasheet, please contact our Technical Support. We unconditionally guarantee our product.
Our team of scientists are ready to help you with expert recommendations or technical assistance related to applications or product use.
Information
© 2026 NeoBiotechnologies. All Rights Reserved.
Monday-Friday: 8:00am – 8:00pm Pacific Time
