Learn about our comprehensive antibody validation methods to ensure monospecificity.  Antibody Validation>>

Human Nucleolar Antigen (Marker For Human Cells) Antibody [NM95]

In Stock
Catalog Number Formulation Size Price
MSM2-95-CF488-100T
Purified Ab conjugated to CF488
0.5ml at 100ug/ml
$519.00
MSM2-95-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
MSM2-95-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
MSM2-95-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Summary

This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb NM95 recognizes an antigen associated with the nucleoli in human cells. It can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.

Product Properties & Targets

Antibody Type
Host
Mouse
Species Reactivity
Isotype / Light Chain
IgG1 /
Cellular Localization
N/A, nucleoli
Gene Name
N/A
Positive Control
Any human cells. Tissues.
Immunogen
Nuclei of myeloid leukemia biopsy cells
Alternate Names
N/A, N/A, Not Known

Database Links

Entrez Gene ID
SwissProt
N/A

Additional Information

Clone
NM95
Chromosome Location
Not Known
Mol. Weight of Antigen
Not Known

Functions

  • N/A

Key References

  • Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137.

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

Reviews

There are no reviews yet.

Be the first to review “Human Nucleolar Antigen (Marker For Human Cells) Antibody”

Your email address will not be published. Required fields are marked *

PARTNERSHIP OPPORTUNITIES

We hold Exclusive rights to 10,000 recombinant and hybridoma antibody products, available for Licensing or Collaboration.

LETS TALK