Immunofluorescent staining of PFA-fixed HeLa cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-mouse IgG-CF488 (green). Counterstained with phalloidin

Flow Cytometric Analysis of PFA-fixed HeLa cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1); followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Acetone-fixed frozen human tonsil stained with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1).

SDS-PAGE Analysis Purified Human Nuclear Antigen Mouse Monoclonal Antibody (235-1). Confirmation of Purity and Integrity of Antibody.

Flow Cytometric Analysis of PFA-fixed Jurkat cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of PFA-fixed K562 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Flow Cytometric Analysis of PFA-fixed MCF-7 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescent staining of PFA-fixed MCF-7 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-mouse IgG-CF488 (green). Counterstained with phalloidin.

Immunofluorescent staining of PFA-fixed K562 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-mouse IgG-CF488 (green). Counterstained with phalloidin

Flow Cytometric Analysis of PFA-fixed K562 cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Immunofluorescent staining of PFA-fixed Raji cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-mouse IgG-CF488 (green). Counterstain is phalloidin

Flow Cytometric Analysis of PFA-fixed Raji cells with Human Nuclear Antigen Mouse Monoclonal Antibody (235-1) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

Printable Datasheet

Human Nuclear Antigen (HNA) (Human Cell Marker) [235-1]

Name: Human Nuclear Antigen (HNA) (Human Cell Marker)
SKU: MSM3-99
Availability: InStock

Monoclonals Mouse Monoclonals Primary Antibodies

Summary

This monoclonal antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.

Product Properties & Targets

Host

Mouse

Species Reactivity

Human

Isotype

IgG1

Cellular Localization

N/A, nuclei

Gene Name

N/A

Mol. Weight of Antigen

70kDa & 80kDa

Immunogen

Nuclei of human myeloid leukemia biopsy cells

Database Links

Entrez Gene ID

Not Known

Unigene

Not Known

SwissProt

N/A

Additional Information

Clone

235-1

Human Gene Symbol

Not Known

Chromosome Location

Not Known

Known Applications & Suggested Dilutions

Flow Cytometry (1-2ug/million cells)
Immunofluorescence (1-2ug/ml)
,Immunoprecipitation (1-2 g/500ug protein lysate)
,Immunocytochemistry (Acetone-fixed cells) (1-2ug/ml for 30 minutes at RT),Immunohistology (Frozen) (1-2ug/ml for 30 minutes at RT),Optimal dilution for a specific application should be determined.

Key References

Rosario Sanchez-Pernaute et. al. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease. Brain. 2008;131(8): 2127 2139.
Glaser R, Lu MM, Narula N, Epstein JA (2002) Smooth muscle cells, but not myocytes, of host origin in transplanted human hearts. Circulation Jul 2;106(1):17-9. Parthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's diseaseParthenogenetic dopamine neurons from primate embryonic stem cells restore function in experimental Parkinson's disease

Storage & Stability

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.