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GP2 (Glycoprotein 2) / ZAP75 Antibody [MSVA-475M]

In Stock
Catalog Number Formulation Size Price
2813-MSM15-P0
Purified Ab with BSA and Azide
20ug
$324.50
2813-MSM15-P1
Purified Ab with BSA and Azide
100ug
$654.50
Flat Rate Domestic: $75 | Orders outside the US - Contact Us for Order Information | Ships next business day
GP2 Antibody in Numerous Tissues (IHC)

GP2 Antibody in Numerous Tissues (IHC)

Pancreatic secretory granule membrane major glycoprotein GP2 Mouse Monoclonal Antibody (MSVA-475M) tested on many normal and cancer tissues. The immunohistochemistry staining in these tissues aligns with the expression data in Human Protein Atlas.

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1:100-1:200
IHC Protocol
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Summary

GP2 (glycoprotein 2), also known as ZAP75, is a 537 amino acid secreted protein. It is an integral membrane protein that is secreted from intracellular zymogen granules and associates with the plasma membrane via glycosylphosphatidylinositol (GPI) linkage. GP2 is cleaved and then released into the pancreatic duct along with exocrine secretions. GP2 binds pathogens such as enterobacteria, thereby playing an important role in the innate immune response. The C-terminus of this protein is related to the C-terminus of the protein encoded by the neighboring gene, uromodulin (UMOD). GP2 is also expressed on the apical plasma membrane of specialized microfold (M) cells among enterocytes and serves as a transcytotic receptor for mucosal antigens. M cells are considered a promising target for oral vaccination against various infectious diseases.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Isotype / Light Chain
IgG2c / Kappa
Cellular Localization
Apical cell membrane, Cell membrane, Endosome, Membrane raft, Secreted, Zymogen granule membrane
Gene Name
GP2
Positive Control
Pancreas: Acinar cells should show a strong immunostaining with apical predominance. Duodenum: Glandular cells of Brunner glands should show a weak to moderate GP2 positivity.
Immunogen
Recombinant fragment of human GP2 protein (around aa 35-179) (exact sequence is proprietary)
Alternate Names
Pancreatic secretory granule membrane major glycoprotein GP2, Pancreatic zymogen granule membrane protein GP-2, ZAP75, Glycoprotein 2 (zymogen granule membrane); GP2; Pancreatic zymogen granule membrane associated protein GP2; Pancreatic zymogen granule membrane protein GP-2; ZAP75

Database Links

Entrez Gene ID
2813
SwissProt
P55259

Additional Information

Clone
MSVA-475M
Chromosome Location
16p12
Mol. Weight of Antigen
59kDa

Related Antibodies

View All GP2 Antibodies

Functions

  • Functions as an intestinal M-cell transcytotic receptor specific of type-I-piliated bacteria that participates in the mucosal immune response toward these bacteria. At the apical membrane of M-cells it binds fimH, a protein of the bacteria type I pilus tip. Internalizes bound bacteria, like E.coli and S.typhimurium, from the lumen of the intestine and delivers them, through M-cells, to the underlying organized lymphoid follicles where they are captured by antigen-presenting dendritic cells to elicit a mucosal immune response.

Key References

  • Fukuoka, S., et al. 1991. Proc. Natl. Acad. Sci. USA 88: 2898-2902.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
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