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Glutamate-Cysteine Ligase Regulatory Subunit Antibody [CPTC-GCLM-1]

In Stock
Catalog Number Formulation Size Price
2730-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
2730-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
2730-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1). Confirmation of Integrity and Purity of Antibody.

SDS-PAGE Analysis of Purified GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1). Confirmation of Integrity and Purity of Antibody.

Western Blot Analysis of A549, A431 & HepG2 cell lysates using GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).

Western Blot Analysis of A549, A431 & HepG2 cell lysates using GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).

Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).

Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).

Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).

Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Cardiovascular, Infectious Disease, Nuclear Marker
Isotype / Light Chain
IgG2a / Kappa
Gene Name
GCLM
Positive Control
A549, HepG2 or HeLa cell lysates. Human muscle and liver tissue.
Immunogen
Recombinant human full-length GCLM protein
Alternate Names
Glutamate | cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate, cysteine ligase modifier subunit, GCLM Antibody, Gamma-ECS regulatory subunit Antibody, GLCLR Antibody, GCS light chain Antibody, GSC light chain Antibody

Database Links

Entrez Gene ID
2730
SwissProt
P48507

Additional Information

Clone
CPTC-GCLM-1
Chromosome Location
1p22.1
Mol. Weight of Antigen
31kDa

Related Antibodies

View All GCLM Antibodies

Key References

  • Tu Z, et al. Arch Biochem Biophys 1998;354:247 254.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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