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Glutamate-Cysteine Ligase Regulatory Subunit Antibody [CPTC-GCLM-1]

Additional information

Catalog Number: 2730-MSM1
Gene:

Reactivity:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
2730-MSM1-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
2730-MSM1-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
2730-MSM1-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
Glutamate-Cysteine Ligase Regulatory Subunit Antibody

SDS-PAGE Analysis of Purified GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1). Confirmation of Integrity and Purity of Antibody.

Glutamate-Cysteine Ligase Regulatory Subunit Antibody - Image 2

Western Blot Analysis of A549, A431 & HepG2 cell lysates using GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).

Glutamate-Cysteine Ligase Regulatory Subunit Antibody - Image 3

Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).

Glutamate-Cysteine Ligase Regulatory Subunit Antibody - Image 4

Formalin-fixed, paraffin-embedded human tonsil stained with GCLM Mouse Monoclonal Antibody (CPTC-GCLM-1).

  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
CPTC-GCLM-1
Isotype
IgG2a
Light Chain
Kappa
Gene Name
GCLM
Positive Control
A549, HepG2 or HeLa cell lysates. Human muscle and liver tissue.
Immunogen
Recombinant human full-length GCLM protein
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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Other Information

Key References
  • Tu Z, et al. Arch Biochem Biophys 1998;354:247 254.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
2730
SwissProt
P48507
Chromosome Location
1p22.1
Mol. Weight of Antigen
31kDa
Alternate Names
Glutamate | cysteine ligase regulatory subunit, GCS light chain, Gamma-ECS regulatory subunit, Gamma-glutamylcysteine synthetase regulatory subunit, Glutamate, cysteine ligase modifier subunit, GCLM Antibody, Gamma-ECS regulatory subunit Antibody, GLCLR Antibody, GCS light chain Antibody, GSC light chain Antibody

Summary

GCLM known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia.

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