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EpCAM / CD326 (Epithelial Marker) Antibody [Cocktail PAN-EpCAM]

Additional information

Catalog Number: 4072-MSM9
Reactivity:

Gene:

Host:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
4072-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
4072-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
4072-MSM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
EpCAM / CD326 (Epithelial Marker) Antibody

Formalin-fixed, paraffin-embedded human breast carcinoma stained with EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).

EpCAM / CD326 (Epithelial Marker) Antibody - Image 2

Western Blot analysis of human lung tissue lysate using EpCAM Mouse Monoclonal Antibody (PAN-EpCAM).

EpCAM / CD326 (Epithelial Marker) Antibody - Image 3

SDS-PAGE Analysis of Purified EpCAM Mouse Monoclonal Antibody (PAN-EpCAM). Confirmation of Integrity and Purity of Antibody

EpCAM / CD326 (Epithelial Marker) Antibody - Image 4

Flow Cytometric Analysis of PFA-fixed MCF-7 cells using EpCAM Mouse Monoclonal Antibody (PAN-EpCAM) followed by goat anti-mouse IgG-CF488 (blue); isotype control (red).

  • Flow
  • IF
  • IHC
  • WB
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Flow Cytometry (Flow) 1-2ug/million cells Flow Cytometry Protocol
Immunofluorescence (IF) 1-3ug/ml
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB) 2-4ug/ml Western Blot Protocol

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Clone
Cocktail PAN-EpCAM
Isotype
IgG1
Light Chain
Kappa
Cellular Localization
Cell junction, Lateral cell membrane, Tight junction
Gene Name
EPCAM
Positive Control
MCF-7 or HT29 cells. Human breast tumor.
Immunogen
Recombinant human EpCAM protein (full-length and fragments)
Storage & Stability
Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Abs purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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5
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Additional information

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VIEW ALL ANTIBODIES

Other Information

Functions
  • May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Plays a role in embryonic stem cells proliferation and differentiation. Up-regulates the expression of FABP5, MYC and cyclins A and E.
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
4072
SwissProt
P16422
Chromosome Location
2q21
Mol. Weight of Antigen
40-43kDa
Alternate Names
Epithelial cell adhesion molecule, Adenocarcinoma-associated antigen, Cell surface glycoprotein Trop-1, Epithelial cell surface antigen, Epithelial glycoprotein, Epithelial glycoprotein 314, KS 1/4 antigen, KSA, Major gastrointestinal tumor-associated protein GA733-2, Tumor-associated calcium signal transducer 1, Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1

Summary

It is a cocktail of four highly specific monoclonal antibodies that recognize extracellular as well as intracellular domains of the epithelial cellular adhesion molecule (EpCAM). It is a 40-43kDa transmembrane epithelial glycoprotein, identified as epithelial specific antigen (ESA), or EpCAM. EpCAM is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas. This epithelial antigen plays an important role as a tumor-cell marker in lymph nodes from patients with esophageal carcinoma otherwise classified as node-negative. Epithelial antigen has also been suggested as a discriminator between basal cell and baso-squamous carcinomas, and squamous cell carcinoma of the skin.

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