E. coli O157 [9.88]

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Summary

This MAb shows specificity to E.coli O157 in a simple ELISA. Escherichia coliare Gram negative bacterium that are commonly found in the lower intestine of warm-blooded organisms (endotherms). Their serological types are determined in combination with somatic antigens (O group: O1-O173) and flagella antigens (H type: H1-H56). TheE. colithat cause intestinal infectious diseases including diarrhea, acute gastritis or colitis are referred to as pathogenicE. coli, which are classified into the following 4 groups according to differences in the mode of pathogenicity; enteropathogenicE. coli(EPEC), enteroinvasiveE. coli(EIEC), enterotoxigenicE. coli(ETEC) and enterohemorrhagicE. coli(EHEC). Although the identification of pathogenicE. colirequires verification of their pathogenicity, pathogenicE. colioften have specific serotypes; therefore, typing of the serogroup and serotype is necessary in screening pathogenicE. coli.

Product Properties & Targets

Host
Species Reactivity
Isotype
IgM
Cellular Localization
N/A
Gene Name
N/A
Mol. Weight of Antigen
Not Known
Immunogen
A crude sonicate of E. coli O157

Database Links

Entrez Gene ID
Unigene
SwissProt
N/A

Additional Information

Clone
9.88
Human Gene Symbol
Not Applicable
Chromosome Location
Not Applicable

Known Applications & Suggested Dilutions

  • ELISA (For coating use Ab at 1-5ug/ml, order Ab without BSA)
  • Immunofluorescence (1-2ug/ml)
  • Optimal dilution for a specific application should be determined.

Key References

  • Park S et al. Enzyme-linked immuno-strip biosensor to detect Escherichia coli O157:H7. Ultramicroscopy 108:1348-51 (2008).

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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