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Desmoglein-3 (Squamous Cell Marker) Antibody [5G11]

In Stock
Catalog Number Formulation Size Price
1830-MSM3-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$229.00
1830-MSM3-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$519.00
1830-MSM3-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$519.00
Flat Rate Domestic: $60 | Orders outside the US - Contact Us for Order Information | Ships next business day
SDS-PAGE Analysis of Purified Desmoglein-3 Monoclonal Antibody (5G11). Confirmation of Integrity and Purity of Antibody

SDS-PAGE Analysis of Purified Desmoglein-3 Monoclonal Antibody (5G11). Confirmation of Integrity and Purity of Antibody

Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-3 Monoclonal Antibody (5G11).

Formalin-fixed, paraffin-embedded human Skin stained with Desmoglein-3 Monoclonal Antibody (5G11).

Applications & Dilutions

Applications Tested Dillution Protocol Note
Flow Cytometry (Flow)
1-2ug/million cells
Flow Cytometry Protocol
Immunofluorescence (IF)
1-3ug/ml
Immunohistochemistry (IHC)
1-2ug/ml
IHC Protocol
30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes
Western Blot (WB)
2-4ug/ml
Western Blot Protocol

Summary

Recognizes a protein of 130kDa, identified as Desmoglein-3 (DSG3). This MAb is highly specific to Desmoglein-3 and does not cross-react with other members of the Desmoglein-family. DSG3 is a calcium-binding transmembrane glycoprotein component of desmosomes in vertebrate epithelial cells. Desmosomes are cell-cell junctions between epithelial, myocardial, and certain other cell types. Currently, three desmoglein subfamily members are identified and all are members of the cadherin cell adhesion molecule superfamily.

Product Properties & Targets

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Species Reactivity
Human
Research Areas
Apoptosis, Autophagy, Developmental Biology
Isotype / Light Chain
IgG1 / Kappa
Cellular Localization
Cell junction, Cell membrane, Desmosome
Gene Name
DSG3
Positive Control
A431 cells. Lung squamous cell carcinoma.
Immunogen
Recombinant human DSG3 extracellular protein fragment
Alternate Names
Desmoglein-3, 130 kDa pemphigus vulgaris antigen, Cadherin family member 6, 130kDa pemphigus vulgaris antigen (PVA); Balding (Bal); Cadherin family member 6 (CDHF6); Desmoglein-3 (DSG3)

Database Links

Entrez Gene ID
1830
SwissProt
P32926

Additional Information

Clone
5G11
Chromosome Location
18q12.1
Mol. Weight of Antigen
130kDa

Related Antibodies

View All DSG3 Antibodies

Functions

  • Component of intercellular desmosome junctions. Involved in the interaction of plaque proteins and intermediate filaments mediating cell-cell adhesion.

Key References

  • Mahoney, MG; Hu, Y; Brennan, D; Bazzi, H; Christiano, AM; Wahl, JK III. Delineation of diversified desmoglein distribution in stratified squamous epithelia: implications in diseases. Exp Dermatol. 2006, 15(2):101-9.
  • Wahl JK III. Generation of monoclonal antibodies specific for desmoglein family members. Hybrid Hybridomics 2002, 21(1):37-44.

PubMed Links

Storage & Stability

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Limitations

This antibody is available for research use only and is not approved for use in diagnosis.

Supplied as

200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Warranty

There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.

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