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CXCR5 (Marker of T Follicular Helper (Tfh) Cells) Antibody [CXCR5/13099]

Additional information

Catalog Number: 643-MSM9
Host:

Reactivity:

Gene:

In Stock

Trial Size Available

Carrier-Free Available

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In Stock
Catalog Number Formulation Size Price
643-MSM9-P1ABX
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
100ug
$559.00
643-MSM9-P1
Purified Ab with BSA and Azide at 200ug/ml
100ug
$559.00
643-MSM9-P0
Purified Ab with BSA and Azide at 200ug/ml
20ug
$259.00
Flat Rate Domestic: $95 | Orders outside the US - Contact Us for Order Information | Ships next business day
CXCR5 Antibody in Immunohistochemistry (IHC (P))

CXCR5 Antibody in Immunohistochemistry (IHC (P))

Formalin-fixed, paraffin-embedded human adrenal gland stained with CXCR5 Mouse Monoclonal Antibody (CXCR5/13099). Inset: PBS instead of primary antibody; secondary only negative control.

CXCR5 Antibody in SDS-PAGE

CXCR5 Antibody in SDS-PAGE

SDS-PAGE Analysis of Purified CXCR5 Mouse Monoclonal Antibody (CXCR5/13099). Confirmation of Purity and Integrity of Antibody.

  • IHC
  • Documents
  • Applications & Dilutions
  • Product Properties
  • other information

Documents

Document Name
Datasheet Download Here
Material Safety Data Sheet Download Here

Applications & Dilutions

Applications Tested Dilutions Protocol Note
Immunohistochemistry (IHC) 1-2ug/ml IHC Protocol 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes

Product Properties

Antibody Type
Mouse Monoclonals
Host
Mouse
Applications
Immunohistochemistry
Species Reactivity
Human
Clone
CXCR5/13099
Isotype
IgG2b
Light Chain
Kappa
Cellular Localization
Cell membrane
Gene Name
CXCR5
Positive Control
Human placenta, brain, lung, transitional cell carcinoma, testis, ovarian serous carcinoma or hepatocellular carcinoma.
Immunogen
Recombinant fragment of human CXCR5 protein (exact sequence is proprietary)
Storage & Stability
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Supplied as
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Limitations
This antibody is available for research use only and is not approved for use in diagnosis.

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VIEW ALL ANTIBODIES

Other Information

Functions
  • Cytokine receptor that binds to B-lymphocyte chemoattractant (BLC). Involved in B-cell migration into B-cell follicles of spleen and Peyer patches but not into those of mesenteric or peripheral lymph nodes. May have a regulatory function in Burkitt lymphoma (BL) lymphomagenesis and/or B-cell differentiation.
Key References
  • Wang, Binhan et al. “CXCL13-CXCR5 axis: Regulation in inflammatory diseases and cancer.” Biochimica et biophysica acta. Reviews on cancer vol. 1877,5 (2022): 188799. doi:10.1016/j.bbcan.2022.188799
  • Hussain, Muzammal et al. “CXCL13/CXCR5 signaling axis in cancer.” Life sciences vol. 227 (2019): 175-186. doi:10.1016/j.lfs.2019.04.053
  • Zhao, J., et al. 2022. Activation of CXCL13/CXCR5 axis aggravates experimental autoimmune cystitis and interstitial cystitis/bladder pain syndrome. Biochem. Pharmacol. 200: 115047.
  • Noël, Grégory et al. “Functional Th1-oriented T follicular helper cells that infiltrate human breast cancer promote effective adaptive immunity.” The Journal of clinical investigation vol. 131,19 (2021): e139905. doi:10.1172/JCI139905
  • Li, Jiang-Ping et al. “PD-1+CXCR5-CD4+ Th-CXCL13 cell subset drives B cells into tertiary lymphoid structures of nasopharyngeal carcinoma.” Journal for immunotherapy of cancer vol. 9,7 (2021): e002101. doi:10.1136/jitc-2020-002101
PubMed Links
Warranty
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Entrez Gene ID
643
SwissProt
P32302
Chromosome Location
11q23.3
Mol. Weight of Antigen
56kDa
Alternate Names
C-X-C chemokine receptor type 5, Burkitt lymphoma receptor 1, Monocyte-derived receptor 15, BLR1; Burkitt lymphoma receptor 1, GTP binding protein (chemokine (C-X-C motif) receptor 5); CD185 antigen; Chemokine receptor 5; CXC chemokine receptor type 5; MDR 15; MDR15; MGC117347; Monocyte derived receptor 15

Summary

Burkitt s lymphoma receptor 1 (Blr1) is a lymphocyte specific chemokine receptor expressed at low levels in secondary lymphoid tissues and in defined structures of the cerebellum. The G protein-coupled receptor has significant homology to other chemokine receptors. Stimulation of Blr1 by its ligand, B-lymphocyte chemoattractant (BLC) results in an influx of calcium into the cell and the chemotaxis of the cell. Blr1 is required for B-cell migration into splenic and Peyer s patch follicles. BLC expression in Peyer s patches is highest in germinal centers, where B cells undergo somatic mutation and affinity maturation.

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